HPV DNA与E6 E7 mRNA检测对宫颈病变诊断价值比较研究.docVIP

HPV DNA与E6 E7 mRNA检测对宫颈病变诊断价值比较研究 　　[摘要] 目的 评价高危HPVE6/E7mRNA在宫颈病变中的临床价值。 方法 将70例低度宫颈病变（慢性宫颈炎），子宫颈上皮内瘤变（CINI级）和78例高级别宫颈病变（包括CINⅡ级CINⅢ级和宫颈浸润性癌）分为两组。低度宫颈病变为对照组，高级别宫颈癌组为实验组。 分别进行HPV DNA分型和HPV E6/E7 mRNA检测。结果 低度子宫颈病变和高度子宫颈病变的阳性率分别为14.2%和79.5%。HPV DNA分型检测结果，低度子宫颈病变和高度子宫颈病变的阳性率分别为35.7%和85.7%。高风险HPV E6/E7 mRNA检测特异性，阳性预测值，阴性预测值（85.8%，86.1%，83.5%）显着高于（64.3%，70.5%，71.4%），差异有统计学意义。 结论 高危HPV E6/E7 mRNA检测有效降低了临床诊断中的过度检查和过度治疗的机会，避免了患者因高频重复感染引起的经济负担和精神压力。 　　[关键词] 人乳头瘤状病毒HPV；HPV DNA检测；HPV E6/E7检测；宫颈癌；方法对比 　　[中图分类号] R737.33 [文献标识码] A [文章编号] 2095-0616（2017）12-19-04 　　Comparative study of diagnostic value of HPV DNA and E6 / E7 mRNA detection in cervical lesions 　　LIANG Haiyan CHEN Xiaohong 　　Reproductive Medicine Center， the First Affiliated Hospital of Shantou University Medical College， Shantou 515031， China 　　[Abstract] Objective To evaluate the clinical value of high risk HPVE6/E7 mRNA in cervical lesions. Methods 70 patients with lower-grade cervical lesions （chronic cervicitis， cervical intraepithelial neoplasis， CINI-grade） and 78 patients with high-grade cervical lesions （including CINII grade， CINⅢ grade and cervical invasive carcinoma） were divided into two groups. Lower-grade cervical lesions were set as the control group while high-grade cervical lesions were set as the experimental group. They were respectively given HPV DNA typing and HPVE6/E7 mRNA detection. Results Detection of high risk HPVE6/E7 mRNA. Positive rates of lower-grade cervical lesions and high-grade cervical lesions were respectively 14.2% and 79.5%. Detection results of HPV DNA typing. Positive rates of lower-grade cervical lesions and high-grade cervical lesions were respectively 35.7% and 85.7%. Specificity， positive predictive value and negative predictive value of high risk HPVE6/E7 mRNA detection were respectively 85.8%， 86.1% and 83.5%， significantly higher than those 64.3%， 70.5% and 71.4%. Difference was statistically significant. Conclusion High-risk HPVE6/E7 mRNA detection can effective