冬虫夏草及虫草制品中活性成分检测方法的建立及含量测定-食品科学与工程专业论文.docx

草片制品中测得含有虫草素34.021 mg/g；北虫草中除虫草素外另4种核苷类成分 的总含量最高，达3.96 mg/g，云南产冬虫夏草的含量最低为2.24 mg/g。麦角甾 醇的含量在北虫草中含量最高，约为西藏产冬虫夏草2倍、青海产冬虫夏草3倍。 西藏冬虫夏草所含虫草酸最多，达到159.886 mg/g，且4者虫草酸含量两两间均差 异极显著。4种虫草样品在氨基酸总含量上均为差异显著，其中云南冬虫夏草的 氨基酸总量最高，为29.302 g/100g。云南和西藏冬虫夏草中镁(Mg)含量差异不显 著，含量为4种虫草样品中最高，分别为1.554mg/g和1.544 mg/g，铜(Cu)的含量 在青海冬虫夏草中最高，达到20.127 mg/kg，西藏冬虫夏草含铁含量最多，是 791.653 mg/kg，而锌(Zn)和锰(Mn)则在云南冬虫夏草中含量最大，分别为145.589 mg/kg和64.359 mg/kg。虫草菌丝体口服液中未测出含有肌苷、腺嘌呤、虫草素、 麦角甾醇、虫草酸和氨基酸成分。仅在虫草片制品中测到含有虫草素，其含量为 34.021 mg/g，麦角甾醇在胶囊制品中的含量较虫草片的含量高，达到1.983 mg/g， 且胶囊制品中氨基酸总量约为虫草片制品氨基酸总量的2倍，达31.164 g/100g， 虫草酸在虫草片制品中的含量比胶囊制品要高，达162.591 mg/g，在所有测定的 成分中，口服液的含量均是最低的。 3、按国标对所有样品中总砷(As)及铅(Pb)、镉(Cd)的含量进行测定。实验结 果表明：重金属铅和镉分别在西藏和云南产冬虫夏草中含量最多，铅在西藏冬虫 夏草中含量为211.395 μg/kg，镉在云南冬虫夏草中含量为29.008 μg/kg，西藏总砷 含量最多为4.864 mg/kg，三种有害金属的含量均在北虫草中含量最低。制品中虫 草片的铅和镉含量比另外两种制品的含量要高，分别为13.174 μg/kg和18.838 μg/kg，口服液中未测到有铅存在，且另两种有害金属的含量均为最低。 关键词：冬虫夏草，北虫草，核苷类，虫草酸，氨基酸，金属，检测  Establishment of Detection Methods and Determination of Contents of Active Components in Cordyceps and Related Products ABSTRACT Cordyceps sinensis is a species of edible and medicinal fungi that is scarce and useful. It is considered as nutritional and healthy products because of its special function in anticancer and antivirus. The growing districts of Cordyceps are mainly in high altitude and cold area in China, such as Tibet, Yunnan provinces and so on, so it is hard to be artificially cultivated, and its price increases very high. As a lot of fake Cordyceps appeared in the market, it’s urgent to develop efficient analytical methods to evaluate related products. The content of five nucleosides, cordycepin, ergosterol, amino acid and metal elements of three Ophiocordyceps sinensis samples and one Cordyceps militaris sample collected from three provinces and three related products were detected in this experiment, and the results would be helpful to monitor and control the qualities of Cordyceps. The main results of this paper are summarized as follows: A determination method for