短时间低氧处理对创面愈合的影响及其机制研究-外科学(烧伤)专业论文.docxVIP

TBS tris-buffered saline 基甲烷盐缓冲液 三羟甲基氨基 甲烷盐缓冲液 TEMED PMSF Tween-20 Tris EMT MET HIF1-α mRNA PH N,N,N,N’-tetramethylethylene diamine phenylmethyl sulfonylfluoride polyethylene glycol sorbitan monolaurate trihydroxymethylaminomethane epithelial-mesenchymal transition mesenchymal-epithelial transition hypoxia-inducible factor 1α messenger ribonucleic acid Potential of hydrogen 四氨基乙二胺 蛋白酶抑制剂 吐温-20 三羟甲基氨基甲烷 上皮-间充质转化 间充质-上皮转化 缺氧诱导因子 1α 信使 RNA 酸碱度 DAPI 4,6-Diamidino-2-phenylindole 4,6-二脒基-2-苯基吲哚 mTOR mammalian target of rapamycin 哺乳动物雷帕霉素靶蛋白 2 The effect of short-time hypoxia on wound healing and its mechanism * Abstract Background and purpose Skin is of great importance in maintaining body’s stablity, preventing it from physical, chemical, mechanical damage and invasion of pathogenic microorganisms. In serious burn patients, the integrity of skin is severely destroyed, which leads to loss of barrier function, besides, wound infection can provide an important source of systemic infection. Treatment of burn wounds can significantly affect patients condition, that is why we emphasize the priority for the restoration of an intact skin barrier, also, known as re-epithelialization. During this process, the migration of keratinocyte is not only the initial event, but also the rate-limiting step[1], while their proliferation can meet the need of migration. Meanwhile, epithelial-mesenchymal transition of keratinocyte results in the increase of motility, which is propitious to wound repair. It’s widely known that hypoxia can occur locally and systemically after severe burn. Cardiogenic shock brings sharp cutoff of effective circulating blood volume and tissue hypoperfusion. On the basis of hypoperfusion, local vascular damage, wound infection and respiratory burst contribute to the hypoxia environment of wound. In clinical application, not only hypoxia environment formed by dressing and vacuum suction can accelerate wound healing[2-4], but also hyperbaric oxygen therapy[5-7], whi