La抑制Hela细胞生长的作用机制的研究.docVIP

La抑制Hela细胞生长的作用机制的研究 　　[摘要] 目的:观察La(C7H5O3)2?(C9H6NO)对体外培养的Hela细胞的生长抑制作用,并进一步探讨其作用机制。方法:应用MTT法检测细胞增殖活性,流式细胞仪检测细胞周期分布,AnnexinⅤ-FITC染色法检测细胞凋亡率。结果:不同浓度的La(C7H5O3)2?(C9H6NO)处理Hela细胞24～96 h后,细胞增殖受到显著抑制,并呈浓度及时间依赖性;0.5、1.0、5.0、10.0 μmol/L La(C7H5O3)2?(C9H6NO)处理72 h后,Hela细胞G0/G1期细胞数量显著增加,S期细胞数量显著减少(P0.01);各浓度组细胞凋亡率均显著增加(P0.01)。结论:La(C7H5O3)2?(C9H6NO)对Hela细胞具有生长抑制作用,其机制与阻滞细胞周期及诱导凋亡有关。 　　[关键词] La(C7H5O3)2?(C9H6NO);Hela细胞;凋亡 　　[中图分类号] R329.2+8[文献标识码]A [文章编号]1673-7210(2010)05(c)-005-03 　　 　　Study on the inhibitory mechanism of La(C7H5O3)2?(C9H6NO) in the proliferation of Hela cells 　　ZHANG Hui1, YU Liping2, LI Xu1, HU Jilin1, HE Zhixiong3, LIN Yingbiao3, LIU Qiaotu3, LI Qiangguo1 　　(1.Department of Pharmacy of Chemistry and Life Science, Xiangnan University, Chenzhou 423000, China; 2.Library of Xiangnan University, Chenzhou 423000, China; 3.Department of Laboratory, the First Peoples Hospital of Chenzhou, Chenzhou 423000, China) 　　[Abstract] Objective: To investigate the inhibitory effect of La(C7H5O3)2?(C9H6NO) in the proliferation of Hela cells, and explore its mechanism. Methods: MTT assay, flow cytometry and AnnexinⅤ-FITC staining were respectively performed to determine the cell proliferation activity, cell cycle and apoptotic rate. Results: After treated with different concentrations of La(C7H5O3)2?(C9H6NO) for 24 to 96 hours, the proliferation activity of Hela cells was inhibited in a dose- and time-dependent manner. Hela cells were treated with 0.5, 1.0, 5.0, 10.0 μmol/L of La(C7H5O3)2?(C9H6NO), the number of cells in G0/G1 phase significantly increased and the number of cells in S phase significantly increased in cells treated with different concentrations of La(C7H5O3)2?(C9H6NO) (P0.01). Conclusion: La(C7H5O3)2?(C9H6NO) can inhibit the growth of Hela cells by blocking cell cycle and inducing apoptosis. 　　[Key words] La(C7H5O3)2?(C9H6NO); Hela cell; Apoptosis 　　 　　La(C7H5O3)2?(C9H6NO)是将七水氯化镧与水杨酸、8-羟基喹啉反应合成的镧与水杨酸、8-羟基喹啉三元混合配合物,为一种人工合成的稀土配合物。水杨酸是常用的抗感染药物[1],喹