对化湿行瘀清热方剂治疗口腔扁平苔藓前后血清中差异蛋白的研究-口腔医学专业论文.docxVIP

万方数据 万方数据 目 录 中文摘要·················································································1 英文摘要·················································································3 英文缩写·················································································5 研究论文 对化湿行瘀清热方剂治疗口腔扁平苔藓前后血清中差 异蛋白的研究 前言·················································································6 材料与方法········································································8 结果···············································································17 附图···············································································19 附表···············································································22 讨论···············································································23 结论···············································································26 参考文献·········································································27 综述 口腔扁平苔藓的蛋白质组学研究及发病机制的探讨················· 29 致谢·····················································································38 个人简历···············································································39 中 中 文 摘 要 PAGE 10万方数据 PAGE 10 万方数据 对化湿行瘀清热方剂治疗口腔扁平苔藓前后 血清中差异蛋白的研究 摘 要 目的：对口腔扁平苔藓(oral lichen planus,OLP)患者使用化湿行瘀清热 方剂前、后血清中的差异蛋白进行研究，探讨化湿行瘀清热方剂对口腔扁 平苔藓的治疗效果及相关作用机制。 方法： 1 抽取 OLP 患者初诊和经化湿行瘀清热方剂治疗 8 周后的静脉血， 静置 30min 后离心，吸取上层血清，苯酚抽提法提取总蛋白，Bradford 法 测 定 蛋 白 浓 度 ， 采 用 双 向 荧 光 差 异 凝 胶 电 泳 技 术 (two-dimensional fluorescence difference gel electrophoresis,2-D DIGE)筛选治疗前、后的差异 蛋白。 2 液相色谱-质谱联用技术(liquid chromatography-mass spectrometry， LC-MS)鉴定由 2-D DIGE 筛选出的 OLP 患者治疗前、后血清中的差异蛋 白点。 3 蛋白免疫印迹法(Western blot)验证 OLP 患者治疗前后血清中差异 蛋白的表达情况。 4 酶联免疫吸附试验(ELISA)对 OLP 患者治疗前后血清中差异蛋白 的表达情况进行定量研究。 结果：1 口腔扁平苔藓患者经过化湿行瘀清热方剂治疗前、后的血清 中存在差异蛋白点，质谱技术对差异蛋白点进行验证，筛选出的差异蛋白 有结合珠蛋白、人抗凝血酶Ⅲ、补体成分 C1、维生素 D 结合蛋白；2 Western blot 结果显示结合珠蛋白的表达量在口腔扁平苔藓患者治疗后的血清中 较治疗前高,人抗凝血酶Ⅲ 在治疗后的血清中表达量较治疗前低 (P＜ 0.05)；3 ELISA 结果显示结合珠蛋白在口腔扁平苔藓患者治疗后的血清中 浓度较治疗前