肠易激综合征内脏感觉过敏与血瘀相关性研究-药理学专业论文.docxVIP

成都中医药大学硕士学位论文 成都中医药大学硕士学位论文 PAGE PAGE 1 摘 要 目的: 研究肠易激综合征内脏感觉过敏模型大鼠血液流变性和血管内皮功 能变化，探索肠易激综合征内脏感觉过敏与血瘀症的相关性。方法：30 只新生 雌性 SD 大鼠，随机分为对照组、模型组和益母草组，每组 10 只，模型组和益母 草组大鼠在出生后的第 8 天至 21 天，每天接受结直肠刺激，于出生后第 35 天观 察大鼠的腹部回缩反射以进行肠道敏感性评估。第 36-63 天连续给药 4 周，益母 草组大鼠灌胃给予剂量为 3 g/kg/日的益母草颗粒药液，对照组和模型组灌胃等 体积纯化水。于出生后第 64 天再次评估肠道敏感性，并检测血液流变性、血清 内皮素、一氧化氮和去甲肾上腺素含量。结果：1、在第 35 天、第 64 天模型组 大鼠引起腹部抬起和背部拱起的压力阈值均较对照组降低，两组相比有显著性差 异(P0.01)。 2 、模型组大鼠的全血粘度（切变率为 1/200s、1/30s、1/5s、 1/1s）、血浆粘度、红细胞压积、全血高切相对指数和纤维蛋白原含量比对照组 明显或显著升高（P0.05 或 P0.01)，PT、APTT 则显著减少（P0.01)。 3、模 型组大鼠血清内皮素、去甲肾上腺素含量比对照组显著升高（P0.01），血清一 氧化氮含量显著降低（P0.01）。 4、与模型组比较，益母草组的压力阈值显著 升高(P0.01)；全血粘度（切变率为 1/30s、1/5s、1/1s）、血浆粘度、红细胞 压积、纤维蛋白原含量明显或显著降低（P0.05 或 P0.01)，PT、APTT 显著增 加（P0.01)；血清内皮素、去甲肾上腺素含量显著降低（P0.01），血清一氧化 氮含量显著升高（P0.01）。结论：1、肠易激综合征内脏感觉过敏大鼠有血液流 变性和血管内皮功能的异常改变，肠易激综合征内脏感觉过敏与血瘀相关。益母 草“以药探证”反证了这一点。2、血清去甲肾上腺素升高可能在肠易激综合征 血瘀证形成过程中起重要作用。 关键词：肠易激综合征；内脏感觉过敏；血瘀；血液流变性；血管内皮功能 Abstract Study of blood stasis on the rat model of visceral hypersensitivity of irritable bowel syndrome Objective:Irritable bowel syndrome (IBS) is a common gastrointestinal diagnosis .The symptoms of IBS are similar to those of blood stasis. The purpose of this study was to investigate changes of hemorheology and endothelium function on the model of visceral hypersensitivity of IBS in rats, and to assess the relationship between blood stasis and visceral hypersensitivity of IBS. Methods: 30 neonatal female SD rats were randomly divided into three groups: model group, control group and Herba Leonuri group. The models and Herba Leonuri group received mechanical colonic stimulation between postnatal days 8 and 21.The threshold of abdominal withdrawal reflex (AWR) were recorded during colorectal distention at postnatal 35th day. Then Herba Leonuri group were treated by traditional Chinese herb Herba Leonuri(with dosage of 3.0 g/kg)for 4 weeks. Models and controls were given purified water respectively. The threshold of AWR were recorded again at postnatal 64th day. At t