产氢产乙酸互营共培养体的筛选及特性研究微生物学专业论文.docxVIP

哈尔滨工业大学理学硕士学位论文 哈尔滨工业大学理学硕士学位论文 Abstract It is known that methanogensis is the limited step in the methane fermentation process. Some studies indicated the ecological niche of syntrophic acetogens is narrower than methanogens and more sensitive to environment. Additional, the metabolism of syntrophic acetogens restrict directly the growth and metabolism ability of methanogens in the anaerobic digestion system. In another word, the acetogensis is the first rate-limited step in anaerobic digestion. There are a few reports on syntrophic acetogen bacteria and several pure cultures have obtained under certain conditions. However, little study has carried out on improving the efficiency of anaerobic digestion by enhancing the function of acetogens due to the difficulty in purity and ampliative culture. Then, the isolation of syntrophic acetogen cocultures may be a better choice in view of the difficulties mentioned above. In order to overcome the difficulty in recovery of syntrophic acetogen bacteria, the medium and the culture condition were improved and two syntrophic acetogen co-cultures, 7-m-2a and 11-O-1, were obtained. Cultured in selective medium with propionic acid and butyric acid as carbon source for 30 days, the acetic acid yields for 7-m-2a and 11-O-1 were 2385 mg/L and 3061 mg/L with a hydrogen yield of 447.50 mL/L-culture and 484.82 mL/L-culture respectively. The population structures of 7-m-2a and 11-O-1 were made clear by polymerase chain reaction - denaturing gradient gel electrophoreses (PCR-DGGE) analysis. Seven bandings were showed in the DGGE profile of 7-m-2a and nine in 11-O-1’ profile, indicating that two or more bacteria existed in 7-m-2a and 11- O-1. The 16S rDNA sequences of all the clones from the 16 bandings showed that both 7-m-2a and 11-O-1 were new syntrophic acetogen cocultures with non- methanogens as the associated bacteria. The associated bacteria are uncultured bacterium 054E12_B_DI_P58 and Sedimentibacter sp. JN18_A14_H