草鱼卵泡抑素(Follistatin)重复基因的克隆和功能研究-动物遗传育种与繁殖专业论文.docxVIP

上海海洋大 上海海洋大学硕士学位论文 PAGE PAGE 2 containing ten cysteine groups. When compared to human and zebrafish, grass carp fst-1 and fst-2 have more than 85% similarity in the coding region. Both fst-1 and fst-2 mRNAs were detected by RT-PCR throughout the embryogenesis and showed differential expression patterns. In the normal state, the grass carp embryonic development in various stages of the fst-1 expression level is more constant and the difference was not significant. The fst-2 until 16hpf when they begin to express, but the expression level was gradually decreased from 16hpf to 40hpf.By in situ hybridization, fst-1 mRNAs was observed in in the brain, hindbrain and skeletal muscle at the outer edge of the section expression, whereas fst-2 mRNAs were expressed mainly in the brain and skeletal muscle. In adult fish, fst-1 mRNAs were transcribed in multiple tissues except in gill and intestine, while fst-2 mRNAs were detected mainly in eyes and slow-swtich muscle. Further through microscopic injection fst-2 gene in the grass carp embryos, the figure is there any significant change.It can lead to grass carp embryos in the back to the yolk sac bending and tails become warped. When excess fst-1 gene expression of grass carp have obvious no shape change. In this study, Tgf2 transposon mediates efficient transposition of the Fsts gene in carp, grass carp, bream. Bream transgenic group compared with the control group length has been increased, the individual variable larger. Preliminary results indicate that certain feasibility by increase Fsts protein to improve animal muscle production. Our results suggest that duplicated Fsts genes may play conserved and divergent roles in regulating grass carp growth and development. KEY WORDS: Ctenopharyngodon idellus, Fst, Sequences analyse, RT-PCR, Whole-mount in situ hybridization, Microscopic injection 目 录 HYPERLINK \l _bookmark0 引 言 1 HYPERLINK \l _bookmark1 第一章 草鱼卵泡抑素重复基因 Fsts 的克隆与功能研究 3 HYPERLINK \l _bookmark2 1.1 实验材料与方法 3 HY