草鱼恒定链基因的克隆、分子特征及其与MHC分子关系的研究-预防兽医学专业论文.docxVIP

万方数据 万方数据 Abstract Invariant chain (Ii) is a nonpolymorphic type II transmembrane glycoprotein, which encodes the leukocyte differentiation antigen CD74 and expresses on some cell surface. As a chaperone of MHC class II molecules, Ii plays a auxiliary effect on MHC II molecule correct folder, assembling and polymerization, and forms a nonamer with MHC II molecules into in the endoplasmic reticulum and targets the complexes to endosome body. The CLIP region (the class-II associated Ii chain peptide) of invariant chain occupies the antigen binding groove of MHC II molecules, which prevents endogenous antigen peptide from associating with the MHC II class of molecules. So Ii plays an importnat role in the process which MHC class II molecule present exogenous antigen to CD4+ T cells and has an important effect on immune reaction. Ii is currently based on DNA vaccine that has been related applications. Currently, the research of structure and function of invariant chain focuses on the human and other mammals as well as birds, the research of fish is in the lagging stage. Ii of fish is probably the most primitive structure model. To study the structure and function of fish Ii, we firstly cloned grass carp Ii gene by RT-PCR and RACE and analyzed bioinformatics function and structural model, while the relationship of the grass Ii and MHC quantitative expression in different tissues and the characteristics of cellular localization expression were also studied. Additionally the antibodies to grass carp invariant chain were prepared, which provided a theoretical basis for further study of structure, function and the evolutionary relationship of the invariant chain of fish as well as the relationship between the major histocompatibility complex and invariant chain. First, we successfully cloned and sequenced first time Ii gene of grass carp by RT-PCR and RACE (rapid amplification of cDNA ends). The full length Ii of grass carp was 1116 bp, the length of open reading frame wa