萼花臂尾轮虫混交雌体繁殖生物学研究-生态学专业论文.docxVIP

时之间也无显著差异。性比对青岛品系轮虫的混交雌体受精率有显著的 影响，但对芜湖品系无显著影响；在性比为 1：4、1：2 和 4：1 时，两 品系轮虫的受精率间无显著差异；而在性比为 1：1 和 2：1 时，青岛品 系轮虫的受精率均高于芜湖品系。当年龄在 4~16h 以内时，芜湖品系轮 虫混交雌体的受精率随其年龄的增大而非线性下降，12h 之后皆不再可 受精，半受精龄长(LF50)为 5.32h。芜湖品系雄体的受精能力随着其年龄 的增大而非线性降低，半受精龄长(LF50)为 15.05h。 3、运用单个体培养方法，以浓度分别为 2.0、5.0 和 8.0×106cells/mL 的斜生栅藻为饵料，对采自青岛和芜湖两地的萼花臂尾轮虫未受精的和 受精的混交雌体的生活史特征进行了研究。结果表明，食物浓度仅对青 岛品系轮虫未受精的混交雌体的繁殖后期历时和卵体积有显著的影响， 但对芜湖品系轮虫未受精的混交雌体的繁殖前期历时、平均寿命、卵体 积和生殖投入均具有显著的影响；青岛品系轮虫受精的混交雌体的繁殖 前期历时、卵体积和生殖投入显著地受食物浓度的影响，而芜湖品系除 了繁殖后期历时外，其它各生活史参数均显著地受食物浓度的影响。在 低食物浓度(2.0×106 cells/mL)、中等食物浓度(5.0×106cells/mL)和高食 物浓度(8.0×106cells/mL)下，两品系轮虫未受精的和受精的混交雌体在 诸多生活史参数上均具有显著差异。 关键词： 萼花臂尾轮虫；混交雌体；地理品系；交配行为； 受精作用；生活史特征 Studies on Reproductive Biology of Mictic Females in Freshwater Rotifer Brachionus calyciflorus Abstract Brachionus calyciflorus is among the cosmopolite species of freshwater rotifers. It plays an important role in the material cycle and energy transportation of freshwater ecosystem. Furthermore, it was found quite valuable in aquaculture and environmental monitoring. This article concentrated on the effect of food concentration on the initiation and the life history traits of micitc females in B. calyciflorus, as well as the comparative study on the mating behavior and fertilization of mictic females in two strains. The main contents were presented as the following: The effects of food concentration on the initiation of mictic females in the B. calyciflorus populations were studied by means of population accumulative culture. The results showed that when the food concentration was 2.0 × 106cells/mL, 5.0×106cells/mL and 8.0×106cells/mL, the emerging time of male egg- producing mictic females, males and resting egg-producing mictic females were 0.5, 1.5 and 2d，0.5, 1.5 and 3d, and 0.5, 1, and 1.5d, respectively. During the 10-day- culture, the mean density and the production of amictic females and male egg-producing mictic females were both the highest at the food concentration of 8.0 × 106cells/mL, while no signi