从草鱼内脏制取饲用多肽粉的工艺研究-化学工程与技术专业论文.docxVIP

关键词：草鱼内脏；水解产物；抗氧化性；饲用多肽粉；干燥工艺 Abstract Fish oil and the antioxidant activity hydrolysate was respectively extracted from grass carp viscera using hot water autolytic hydrolysis. On the basis of the single factor, the hydrolysis conditions were optimized by response surface methodology (RSM) and the antioxidant activity of hydrolysate were investigated. The optimised hydrolysis conditions were temperature 59℃, heat time 74 min and addition water 56 mL/100 g sample. Under above conditions, the oil extraction rate was 68.34 ± 0.89%, which was in accordance with the predicted value of 71.60%. Compared on antioxidant activity of homogenate that was not hydrolyzed by hot water autolytic method, the hydrolysate of each 10 miligram dry material exhibited a stronger scavenging activities up to (18.68±1.80)% for hydroxyl radical. 1884 g ctenopharyngodon idellus offal was hydrolyzed using above optimising technological parameter, 448 g fish oil, 138 g residue, and 2017 mL hydrolysate (solid content was 10.86%) was prepared. After residue was extracted by petroleum ether, its fat content declined to 17%. Hydrolysate concentrated was dehydrated by employing hot air drying and spray drying. Entire hot air drying process mainly was falling rate period controlled by internal diffusion. The mathematical model was in accord with Page equation: MR=exp(-(0.01371+0.0003234T-0.02672h)t0.7599), the experiment indicated that test value was agreed with simulated value. Parameter of spray drying was as followed: Inlet air temperature was 170~190℃, outlet air temperature was 80~90℃, solid content was 25%~35% and the rate of material entrance was 15~20 mL/min. Antioxidant activity of polypeptide powder obtained from hot air drying and spray drying was discussed respectively. The reducing power of the polypeptide powder got from hot air drying was (0.958 + 0.014) / 1.0 mg/mL, the rate hydroxyl free radical scavenging rate was (54.48 + 0.69) % / 40 mg/mL. while the reducing power of th