仿刺参(Apostichopus japonicus)体腔液的抗菌特性-水产养殖专业论文.docxVIP

显差异，LYZ 可能是仿刺参清除入侵溶壁微球菌的主要免疫因子；灿烂弧菌诱导仿刺参免 疫因子应答变化显著不同于其他 4 株细菌，显示出本研究选取的 5 个免疫指标在预警灿烂 弧菌病害上具有潜在应用价值；同样地，在仿刺参中，停乳链球菌也具有作为免疫增强剂 的潜在应用价值。 关键词：仿刺参；体腔液；抗菌活性；细菌；金属离子 Ⅱ Abstract Apostichopus japonicus belongs to Echinidermata, Holothuroidea, Aspidochirotidae, Stichopodidae, Apostichopus, and it is an economically important aquaculture species. Bacterial disease is one of the primary factors limiting the development of A. japonicus aquaculture. In order to study the antibacterial spectrum of coelomic fluid from A. japonicus and the effects of divalent metal ions on the antibacterial activities of coelomic fluid, using the method of bacterial growth curve determination, we determined the effects of coelomocyte lysate supernatant and coelomic fluid supernatant from A. japonicus on the growth of Vibrio harveyi, Vibrio splendidus, Shewanella baltica, Pseudoalteromonas nigrifacien, Staphyloccocus aureus, Micrococcus lysodeikticus, Streptococcus dysgadysgalactiae, Nocardiopsis sp., respectively, and then determined the effects of Mg2+, Mn2+, Ca2+, Fe2+, Zn2+, and Cd2+ that commonly found in marine environment on the antibacterial activities in A. japonicus coelomic fluid supernatant with M. lysodeikticus as tested bacteria. The results showed that the coelomocyte lysate supernatant had no obvious effects on the growth of all tested bacteria, while the coelomic fluid supernatant only showed strong inhibition on the growth of M. lysodeikticus. In addition, Mg2+ and Zn2+ increased the inhibition of coelomic fluid supernatant on the growth of M. lysodeikticus, while Mg2+, Ca2+, Fe2+ and Cd2+ showed no obvious effects on the antibacterial activities of coelomic fluid supernatant. The above results suggested that A. japonicus coelomic fluid in vitro only had limited-spectrum antibacterial activity, and the immune factors directly related to anti-bacteria were mainly distributed in coelomic fluid supernatant. Besides, these antibacterial factors in coelomic fluid supernat