房颤患者心房肌细胞CX40表达及心房纤维化的研究-心血管内科专业论文.docxVIP

1、电镜观察及免疫组化分析结果均显示心房颤动者心房肌间质纤维化程度明显 加重，提示心房肌间质纤维化参与了房颤的发生与维持。 2、CX40 在房颤组心房肌含量明显降低，且分布不均，表明 CX40 表达下降是目 前房颤的发生与维持的关键的已知因素。 关键词：心房颤动,缝隙连接,纤维化 Study on expression of CX40 in atrial muscle cell and myocardial fibrosis in patients with atrial fibrillation Abstrat Background and objective: Atrial fibrillation is the most frequently encountered sustained cardiac arrhythmia in clinical practice at present and is a great hazard to human health, the incidence rate has gradually improved accompany with age. But its pathogenesis is not conclusive. The investigation of animal models of atrial fibrillation and human surgical specimens of atrial fibrillation has demonstrated that the incidence and maintenance of atrial fibrillation may be realated to myocardial fibrosis and gap junctions. There are five gap junction proteins had been confirmed exist in heart, these are CX31.9, CX37, CX40, CX43 and CX45. In addition, CX40, CX43 and CX45 are mainly distributed in atrial tissue, CX43 is widely distributed in atrial and ventricular muscle cells, as for CX40, it is mainly distributed in atrial and Purkinje conduction system, the content of CX45 is less contrast to other gap junction proteins and its expression in atrial is slightly higher than in ventricular. Myocardial fibrosis is mainly reflected as the increase of collagen fiber deposition in interstitial tissue of myocardium, moreover, in all the collagen fiber in interstitial tissue of myocardium, collagen type I accounts for more than 80%. We collected 25 cases of rheumatic heart disease atrial tissue samples, using transmission electron microscopy and immunohistochemical to study relationship of atrial remodeling, distribution of CX40, the content and distribution of collagen type I with atrial fibrillation and to further explore the mechanism of the incidence and maintenance of atrial fibrillation. Methods: 25 patients with rheumatic heart disease who received thoracotomy were divided into atrial fibrillation group and sinus