蛋白组学在肾脏病研究中的应用资料讲解.pptVIP

蛋白组学在肾脏病研究中的应用;内 容;蛋白质组和蛋白质组学;Proteomics includes not only the identification and quantification of proteins, but also the determination of their localization, modifications, interactions, activities, and, ultimately, their function.;Nephrol Dial Transplant (2010) 25: 11–16;研究过程：蛋白质分离、鉴定以及信息资料分析处理三部分;蛋白质组学重要工具;Two-dimensional Gel Electrophoresis;;质谱技术;表面增强激光解吸电离飞行时间质谱（surface enhanced laser desorption ionization time-of flight mass spectrometry， SELDI-TOF-MS） 通过测量肽段离子相关参数,如飞行时间(TOF),即可计算得到准确的肽段质量 ;Four different types of MS-based proteomic technologies (a) 2DE-MS; (b) SELDI-MS; (c) LC-MS; (d) CE-MS;Four different types of MS-based proteomic technologies (a) 2DE-MS; (b) SELDI-MS; (c) LC-MS; (d) CE-MS; 不同蛋白质组学技术的比较;多维液相色谱-质谱技术 ;新技术;数据分析data mining;内 容;;尿蛋白质组;尿液标本的准备;稳定性/差异性;稳定性/差异性;糖尿病肾病;糖尿病肾病;糖尿病肾病;糖尿病肾病;糖尿病肾病;2-D gel electrophoresis (2-DE) images of urine samples from diabetic nephropathy (DN) patients and healthy controls.;IgA肾病;IgA肾病;肾小球肾炎;肾小球肾炎;狼疮性肾炎;狼疮性肾炎;狼疮性肾炎;Urine SELDI spectra of class IV SLE nephritis flarecycles. (a) The spectra of a whole flare cycle are presentedbetween the 2000 and 10,000 Da region. The LMW urineproteome shows an overall increase in peaks between 2000 and4000 Da as flare approaches, which then decrease during flaretreatment. ;Intrarenal expression of hepcidin. Immunohistochemical staining for hepcidin is shown for renal biopsy material from a normal kidney (CONT), and three patients with class IV SLE nephritis (SLE). The positive cells are infiltrating interstitial leukocytes.;甘露聚糖结合凝集素(mannose binding lectin,MBL) 与造影剂引起的急性肾损伤(CIN)有关 凝集素途径可能参与造影剂致肾损伤 MBL: CIN可能的生物标记物 ;ARF;肾脏纤维化机制研究;结果：TGF-β可致肾小管上皮细胞损伤;结果：磷酸化蛋白富集;结果： iTRAQ-2D-nano-HPLC-MS/MS 分析;结果： 19个表达下调磷酸化蛋白及磷酸化位点;结果： 19个表达上调磷酸化蛋白及磷酸化位点;结果： PANTHER功能分类结果;结果： Western blot 验证磷酸化蛋白差异表达;结果： TGF-β 致肾小管上皮细胞损伤过程中磷酸化蛋白调控网络;结语;结语;结语;谢谢！