天然脑活素的记忆改善效果及其分子机理研究..docVIP

天然脑活素的记忆改善效果及其分子机理研宄 ：吴正治李映红李明陈熳茵杨敏 】目的：研究中药古方强记汤及天然脑活素对 记忆障碍动物模型在记忆改善方面的作用效果；并运用转基 因技术进一步验证天然脑活素对学习记忆分子开关C REB基 因表达的促进作用。方法：将强记汤及天然脑活素分别以 g/kg和g/kg的量每日经口灌胃给予SPF级小鼠，连续5d， 第6天给药lh后制备成记忆障碍模型，第7天给药~lh后分 别用跳台法和避暗法测试记忆成绩；另用不同剂量天然脑活 素水煎液处理大鼠大脑BI 04CNS神经细胞，以荧光素酶方法 检测CREB表达水平。结果：强记汤及天然脑活素水煎液对 动物记忆障碍模型小鼠记忆成绩中的潜伏时间、错误次数与 模型对照组比较均有统计学意义；而且天然脑活素的改善效 果明显优于原方；不同剂量天然脑活素水煎液CREB表达水 平与对照组比较有统计学意义，且有剂量反应关系。结论: 中药古方强记汤及天然脑活素对实验动物被动回避反应能 力的记忆获得、记忆保持及记忆再现均有的改善作用，能显 著增加在B104CNS神经元细胞中CREB基因的表达。 关键词】天然脑活素；记忆障碍；记忆成绩；CREB; 基因表达 Abstract Objective To explo re the effects of e medicine ancient t i on Memory-Enhanc ing Decoction (MED D on memory improv ement of dysmnesia ythe promotion of he expression of 1 earning and memory CREB gene. Method e treated with MED in dose of g/kg a spectively by intr ation for five day d then, the variou models were prepar th Scopolamine (3 ide (120 mg/kg) by njection and 40% a ody wEight) by int ration on the sixt emory result was e traditional Chines compound prescrip ) and optimized ME animal, and verif optimized MED on t molecular switch s The SPF mice wer or optimized MEDnd g/kg perday reagastric administrs continuously. An s dysmnesia mouse ed respectively wi mg/kg), Cyclohexim intraperitoneal i lcohol ( mL/10 g b ragastric administh day. Next day, m stimated by using Step-down Test and Dark-avoiding Tes t after the mice w ere administered f or half or an hour . In addition, rat cereb rum central nervous system (CN S) nerves cell lin e B104 cells were incubated with opt imized MED in diff erent dose of 50 g/mL and 150 g/mL for a certain tim e. Then, we determ ined the expressio n of CREB gene by means of luciferas e enzyme method. Re suits Both of MED and optimized MED improved the memor y results about la tent time and erro r frequency of dys mnesia mousemodeIs significantly com paring with model control group (P 〔 Key Words ) Optimize d Memory-Enhancing Decoction； Dysmne sia； Memory result ； CREB； Gene expre ssion 学习记忆和认知障碍已成为现代生命科学和神经科学 研究的前沿热点之一