妊娠晚期抗病毒免疫阻断治疗对新生儿血清HBV转录体的影响..docVIP

妊娠晚期抗病毒免疫阻断治疗对新生儿血清HBV转录体的影响 ：李艳红，张伟，朱少君，崔云，郭永，王清 苏勤，冯英明 关键词】宫内感染 Influen ceonserumhepatitis Bvirustranscriptsi nnewbornsbyHBIGimm unoblockingtherapy duringthelaterstag eofpregnancy Abst ract 】AIM：Toinvesti gatethesignificanc eofviraltranscript sinearlystagediagn osisofHBVverticalt ransmission,andtoo bservetheinfluence ofHBIGimmunoblocki ngtherapyonserumHB VRNAinnewbornsfrom HBVcarriermothers. METHODS：FortysixHB sAgpositivepregnan twomenweretreatedw ithant iHBVimmunoth erapy(HBIG200IU/4w eeksandLimidazolew asappliedaslinimen tlOmg/week)since26 weekpregnancy. Theo ther23weretakenasc ontrolswithoutanya ntiHBVtherapy. Vira InuclEicacidsweree xtractedfromseraof mothersbeforethera pyandfromseraofnew borns.HBVDNAandRNA  s,includingthefull RNAmolecules,werea lythroughPCRandRTP XprotEInencodingreHBVDNAinnewbornswa s,includingthefull RNAmolecules,werea lythroughPCRandRTP XprotEInencodingre HBVDNAinnewbornswa rmothershadnotbeen gviralRNAwasdetect thenewbornsirrespe mothers. However,in edmothers,serumRNA runcatedtype. CONCL f serumHBVRNAi sgrea gnosisofearlystage sfromHBVcarriermot stranscribedfromHB bornsratherthanbei othertochild,immun esnotinterferewith ion. However,itmayi lengthandtruncated mplifiedrespective CRtargetedtotheHBV gion.RESULTS：Serum sonlyfoundwhenthei treated. Circulatin edinmorethanhalfof ctiveoftreatmentof thenewbornsoftreat wasuniformlyofthet USION：Applicationo tlyhelpfultothedia infectionofnewborn hers. TruncatedRNAi Vtemplatesinthenew ngtransmittedfromm oblockingtherapydo intrauterineinfect nterferewiththeman ifestionofahighrep licative infect ion. Keywords 】 hepatiti sBvirus；transcript；verticaltransmiss sBvirus；transcript ；verticaltransmiss ion； intrauterinein fection； immunother apy 】目的：探讨乙型肝炎病毒转录体在早期诊断HBV 母婴传播中的意义，并观察母亲抗乙型肝炎病毒免疫球蛋白 免疫阻断治疗对新生儿血清HBV转录体的影响.方法：治疗 组46例HBV表面抗原阳性孕妇自孕26wk开始行抗HBV免疫 治疗；另23例HBsAg）阳性孕妇做为对照组未给予任何抗病 毒治疗.从孕妇治疗前血清及其新生儿血清中提取核酸，经 PCR及RTPCR扩增H BVDNA,全长型RNA和顿挫型RN A，琼脂 糖凝胶电泳显示产物，Sout hern杂交进一步验证扩增产物. 结果：IIBVDNA阳性仅存在于未接受免疫治疗的母亲所生新