诱导单性结实罗汉果果实生长发育规律及品质的研究.docVIP

诱导单性结实罗汉果果实生长发育规律及品质的研究 　　[摘要] 对外源激素诱导罗汉果单性结实所获无籽或少籽果实的生长发育规律及品质进行分析研究。采用形态学观测和酶联免疫吸附法（ELISA）对诱导单性结实果实生长发育过程中果实横、纵径、体积及内源激素（IAA，GA，ABA，ZR）含量进行测定的，同时观测成熟果实大小，种子变化和测定罗汉果甜苷含量，并以二倍体授粉与三倍体诱导及授粉组作为实验对照。结果显示，诱导单性结实罗汉果可获得少籽且种子空瘪无种仁果实;果实大小生长发育规律与授粉组相似;果实横，纵径和体积绝对值二倍体诱导组小于授粉组，而三倍体果实则相反。ABA，IAA含量和ABA/GA比值在果实发育中波动明显，单性结实果实内源激素IAA，ABA含量在生长期0～30 d先下降再升高，生长期内IAA，GA含量诱导果实高于授粉果实;罗汉果总苷质量分数为3.08%，罗汉果甜苷Ⅴ1.47%，两含量略低于授粉果实远高于三倍体授粉与诱导果实含量。激素诱导可使罗汉果单性结实得到果形及大小与正常授粉果实接近且少籽的果实，果实品质接近于正常授粉果实。 　　[关键词] 罗汉果;单性结实;生长发育;内含激素含量;罗汉果甜苷 　　Study on exogenous hormones inducing parthenocarpy fruit growth 　　and development and quality of Siraitia grosvenorii 　　HUANG Jie1， TU Dong-ping2， MA Xiao-jun2*， MO Chang-ming3， PAN Li-mei3， BAI Long-hua3， FENG Shi-xin3 　　（1.Guangxi University of Chinese Medicine， Nanning 530001， China; 　　2.Institute of Medicinal Plant Development， China Academy of Medical Sciences and Peking Union Medical College， 　　Beijing 100193， China; 3.Guangxi Botanical Garden of Medicinal Plants， Nanning 503323， China） 　　[Abstract] To explore the growth and development and analyze the quality of the parthenocarpy fruit induced by exogenous hormones of Siraitia grosvenorii. the horizontal and vertical diameter， volume of the fruit were respectively measured by morphological and the content of endogenous hormones were determined by ELISA. The size and seed and content of mogrosides of mature fruit were determined. The results showed that the fruit of parthenocarpy was seedless and its growth and development is similar to the diploid fruit by hand pollination and triploid fruit by hand pollination or hormones. But the absolute value of horizontal and vertical diameter， volume of parthenocarpy fruit was less than those of fruit by hand pollination， while triploid was opposite. The content of IAA， ABA and ratio of ABA/GA was obviously wavy. At 0-30 d the content of IAA and ABA of parthenocarpy fruit first reduced then increased， content of IAA and GA parthenocarpy fruit was higher than tha