传代培养前后内皮细胞差异表达基因消减NA文库的构建..docVIP

传代培养前后内皮细胞差异表达基因消减cDNA文库的构建 ：张萍，山丽梅，肖小河，付强 关键词】内皮，血管/细胞学；抑制消减杂交；动脉 硬化 Abstract 】 AIM： Tocons tructasubtra ctedcDNAlibr aryofdiffere ntiallyexpre ssedgenesinf reshlyisolat edadultbovin eaorticendot helialcells ( FiBEC)andcul turednewborn aorticendoth elialcells (C nBEC). METHOD S：Thesuppres sionsubtract ivehybridiza tion(SSH)was usedtoisolat ecDNAfragmen tsofnonexpre ssedorlowexp ressedgenesi nCnBEC. Thent hesecDNAfrag mentsweredirectlyinserte dintoT/Aclon ingvestortos mentsweredir etupthesubtr activelibrar y. Theamplif iedlibrarywa srandomlypic keda.ndidenti fiedusingres trictionenzy medig