3甲氧基苯甲醛左氧氟沙星酰腙诱导人肝癌Hep3B细胞凋亡的作用机制分析.docx

优秀毕业论文 精品参考文献资料 inhibited by QNT11(Trimethory-benzaldehyde levofloxacin hydzone (S)-N-(3,4,5-trimethoxybenzylidene)-6-fluoro-1,8-(2,1-oxypropyl)-7-(4-methylpiperazin-1-yl)- quinolin-4(1H)-one-3-carboxylic acid hydrazide) at 2.5 μmol·L-1~20 μmol·L-1 in dose and time dependent manners. The approximate the concentration of 50% growth inhibition (IC50) values of QNT11 for 24h was 14.17μmol·L-1 dependent manners, with an IC50 value of 7.67μmol·L - 1 after 48 hours,with an IC50 value of 5.03μmol·L - 1 after 72 hours;The control group cells displayed dispersed and homogeneous fluorescence and human Hep3B hepatocellular carcinoma cell lines treated withQuinolone derivatives displayed the typicalty morphological features of apoptosis.There were apparent nucleic fragmentation and particulate fluorescence; The typical DNA ladder bands were detected by agarose gel electrophoresis in QNT11 treated groups for 24h compared to the controls, the DNA of controls was intact;The TUNEL result shown that the ratio of apoptosic cells increased with concentration of QNT11 increased ,which were in dose-dependent manner,The difference between controls and QNT11 treated groups were statistically significant (p0.05); The result of Western-blot test indicated that the protein expression of Bax、Caspase-9 and Caspase-3 increased in QNT11 treated groups increased compared to the controls, Bcl-2 expression decreased , both Caspase-9 、 Caspase-8 and Caspase-3 active fragment increased, QNT11 induced a concentration-dependent increase of Cytochrome c contents in cytosolic levels , leakage of cytochrome c from mitochondria, Cell cycle proteins CDK1, CyclinB1 expression decreased, and showed a concentration-dependent manner. Conclusions: 1. QNT11 possess high inhibiting activity to proliferation of Hep3B cells ,cause cell cycle arrest at G2-M phase ,The cell proliferation is inhibited by QNT11 in dose and time dependent manners. QNT11 can induce apoptosis of human hepatocarcinoma Hep3B cells significa