泛素蛋白酶体通路在动脉粥样硬化中作用的研究-病理学与病理生理学专业论文.docx

汕头大学 汕头大学医学院博士学位论文 - - PAGE 10 - significantly increased in H or HI vs. N or N（I P 0.05）, while, SOD and CAT were significantly higher in N or NI vs. H or HI (P 0.05), respectively. (4) The activity of 26S was found no significant differences among N, NI and HI, by contrast, 20S was notly more active in H than any other group (P 0.05).(5) HE results showed that the vascular wall was notly thicker and atheroma was apprent in H vs.HI (P 0.05); (6)Immunochemistry showed that Ub/UPC and I Bα/p I Bα were mainly localized in subendothelium and media vascular smooth muscle cells and more positive in HI vs. H, and so was the case in NI vs. N, by contrast, p65 was more positive in H, respectively (P 0.05). (7) Western blots revealed that Ub/UPC, I Bα, and p I Bα were increased in NI vs. N or HI vs H, respectivelty, whereas, p65 in H was higher than any other group (P 0.05), no significant difference was noted among the rest groups (P 0.05). Conclusions: UPS may be functionally active in the process of AS, the activity of 26S was little affected in oxidative stress, while 20S was actively increased. Aspirin can, via the UPS, partly inhibit the activity of 20S or 26S, attenuate the pathogenesis of atheroma formation, attenuate the degradation of I Bα and pI Bα, and lower the expression of p65, indicating that aspirin may have therapeutic effects on AS, and the UPS may be one of the new targets for the prevention or treatment of AS. Key words: ubiquitin-proteasome system; atherosclerosis; aspirin; hypercholesterolemia; 缩略语表 缩写词 英文名称 中文名称 NF- B Nuclear Factor kappa B 核因子 B I Bɑ Inhibitor of Nuclear Factor kappa B 核抑制因子 B pI Bɑ Phosphated I Bɑ 磷酸化核抑制因子 B 26S Proteasome 26 蛋白酶体 26 20S Proteasome 20 蛋白酶体 20 MDA Maleic Dialdehyde 丙二醛 SOD Superoxide Dismutase 超氧化物歧化酶 CAT Catalase 过氧化氢酶 AS Atherosclerosis 动脉粥样硬化 Ub Ubiquitin 泛素 UPS ubiquitin-proteasome system 泛素蛋白酶体通路 E1 Enzyme 泛素活化酶 E2s Enzymes 泛素转移酶 E3 Enzymes 泛素链接酶 UPC ubiquitin/ubiquitin-protein conjugates, 泛素/泛素蛋白连接物 目 录 中文摘要……