磁共振扩散加权成像动态监测血管阻断剂治疗效果的动物实验研究-影像医学与核医学专业论文.docx

广州医科大学硕士学位论文 广州医科大学硕士学位论文 磁共振扩散加权成像动态监测血管阻断剂治疗效果的动物实验研究 3 3 Diffusion-weighted imaging for dynamically monitoring the treatment effect of vascular-disrupting agent：animal experimental study Abstract Objective: To investigate the imaging characteristics of DWI in rabbit models of hepatic VX2 tumors after treatment of a novel vascular disrupting agent (VDA) M410 and to compare with the dynamic pathological changes. Methods: 28 New Zealand white rabbit models bearing VX2 liver tumor were established and then were randomly divided into treated (intra venous injection of M410 at a dose of 25mg/kg every three days) and control (intravenous injection of sterile saline every three days) g roups. Conventional and DW-MRI were acquired in a 3.0-T MR unit at p retreatment, 4 h, d 1,d 4, d 7 and d 14 post-treatment. B values wit h 0, 100, 200, 300, 400, 500, 600, 700, 800 and 900 s／mm2 were chos en during DW-MRI examination. The tumor volume at every time point w as measured and calculated from T2WI image. With the optimal b value, the corresponding ADC images were rebuilt on the workstation, and A DC values were measured. At each time point, 2 rabbits of both group s were randomly chosen to be killed for HE examination and CD34 imm unohistochemical examination. Pathological results and the DWI image s were compared for evaluating the vascular proliferation of the tum or. Finally，appropriate statistical methods were chosen to make sta tistical analysis for tumor sizes, CNR values of tumor to liver pare nchyma and ADC values of the tumor. Result: Relative to rapidly growing tumors in control rabbits, tumors grew slower in the M410 group d 4 to d 14 after treatment（P0.05）. There was PAGE PAGE 10 no significant difference of tumor volume between the two groups at baseline (1076±613 mm3 in treated group vs. 1273±334 mm3 in control group, P 0.05), 4 h (927±472 mm3 vs. 1108±200 mm3, P 0.05) and d 1 (1218±939 mm3 vs. 1308±284 mm3, P 0.05). With time, tumor growth was observed in bot