信号通路调节胆管癌QBC939细胞的迁移和侵袭的研究.docx

优秀毕业论文 精品参考文献资料 目 录 目录 ······································································································ I 中文摘要······························································································· II 英文摘要······························································································ III 第一章 前言 1 第二章 实验材料·····················································································6 细胞株································································································6 主要仪器及设备 ···················································································6 主要试剂·····························································································7 主要溶液的配制 ················································································· 10 质粒与菌种 ······················································································· 11 第三章 实验方法··················································································· 12 感受态细胞的制备与转化 ···································································· 12 细胞培养··························································································· 12 质粒的提取 ······················································································· 12 质粒转染··························································································· 13 Western Blot 检测基因蛋白水平表达 15 细胞总蛋白质的提取··········································································· 15 SDS电泳 15 Western Blot 分析 16 RNA 提取 16 RNA 反转录 17 荧光定量 PCR 实验（Real -Time PCR 实验） 18 引物设计··························································································· 19 细胞划痕实验（Wounded hell) ······························································ 19 细胞侵袭实验(transwell) ······································································ 20 细胞毒性实验（MTT 法） ························································