芳香烃受体及肝脏代谢酶在饮用水有机提取物染毒大鼠肝脏中的表达及意义-劳动卫生与环境卫生学专业论文.docxVIP

万方数据 万方数据 平仅在高剂量组升高（P0.05）；随着染毒剂量的增加，高剂量组 GSTA1 mRNA 和蛋白表达水平比中剂量组有所降低，差异具有统计学意义（P0.05）；CYP1A2 和 GSTs 酶活性在中剂量与高剂量组显著增加，而 CYP2E1 酶活性仅在高剂量组 增加（P0.05）；与中剂量组相比，高剂量组 GSTs 的酶活性则明显降低（P0.05）。 （4）肝脏代谢酶 CYP1A2、GSTs 酶活性与 AhR 蛋白表达水平均呈正相关关系； 染毒大鼠血清 CHE 水平与 AhR 的蛋白表达水平及 CYP1A2、CYP2E1、GSTs 酶 活性均呈正相关关系。结论：（1）在本实验条件下，较高剂量饮用水有机提取 物暴露可活化芳香烃受体 AhR，从而诱导其配体 HSP90 及 ARNT 的转录表达和 翻译表达。（2）芳香烃受体通路的异常上调，调控下游基因代谢酶 CYP1A2 与 GSTA1 的 mRNA 和蛋白表达增强，诱导代谢酶活性升高。（3）肝脏代谢酶活 性异常升高，在对饮用水有机污染物解毒代谢的过程中，诱导自由基和毒性中间 代谢物的产生，破坏细胞结构，造成肝细胞损伤。 【关键词】饮用水有机提取物；芳香烃受体及配体；肝脏代谢酶；细胞色素 1A2；细胞色素 2E1；谷胱甘肽-S-转移酶 III The Expression of AhR and Metabolic Enzymes in Liver of Rats Exposed to Organic Extracts from Drinking Water Major: Occupational and Environmental Health Postgraduate: GUI Xiao-ling Supervisor: Associate professor YANG Guang-hong Professor ZHANG Ai-hua [Abstract]Objective: Using the previous animal model of liver damage in rats which exposed to organic extracts from drinking water, to observe AhR and metabolic enzymes’s expression. Exploring the organic extracts from drinking water’s role of abnormal liver bioconversion in liver damage. Methods: Extracting the organic compounds from the pipeline water from July to September (wet season) in 2012. 50 SD rats were randomly divided into blank group, solvent control group (with corn oil) and 3 exposed to organic extracts from drinking water groups with 10 rats in each group (half male and female). Exposed groups were administered respectivively with organic pollutants extracted from 5, 20, 80 liter pipeline water per kilogram body weight per day. The exposure time lasted 12 weeks. To prepare rats serum, spectrophotometry was measuring the enzyme activities of ALT, AST, CHE and the content of TP, ALB. Total RNA was extracted from liver tissue, real-time quantitative PCR was taken to measure mRNA expression level of AhR, HSP90, ARNT, CYP1A2, CYP2E1, GSTA1. Total protein was extracted from liver tissue, western blot was taken to detect protein expression level of AhR, HSP90,