耳蜗雪旺细胞来源的Wnt1对耳蜗移植干细胞分化的影响及其分子机制研究-耳鼻咽喉科学专业论文.docxVIP

HYPERLINK \l _bookmark0 1.1 实验动物·····························································································28 HYPERLINK \l _bookmark1 1.2 细胞培养试剂·····················································································28 HYPERLINK \l _bookmark2 1.3 主要仪器·····························································································29 HYPERLINK \l _bookmark3 2 方法 ···········································································································29 HYPERLINK \l _bookmark4 2.1 嗅球神经干细胞的分离、培养·························································30 HYPERLINK \l _bookmark5 2.2 嗅球神经干细胞的鉴定·····································································30 HYPERLINK \l _bookmark6 2.3 耳蜗干细胞移植实验动物分组·························································30 HYPERLINK \l _bookmark7 2.4 ouabain 局部给药建立螺旋神经元损伤动物模型 31 HYPERLINK \l _bookmark8 2.5 耳蜗神经干细胞移植·········································································31 HYPERLINK \l _bookmark9 2.6 免疫荧光染色·····················································································31 HYPERLINK \l _bookmark10 2.7 神经元计数·························································································31 HYPERLINK \l _bookmark11 2.8 统计学分析·························································································32 HYPERLINK \l _bookmark12 3 结果 ···········································································································32 HYPERLINK \l _bookmark13 3.1 神经干细胞的培养与鉴定·································································32 HYPERLINK \l _bookmark14 3.2 神经干细胞移植后的耳蜗形态学观察·············································32 HYPERLINK \l _bookmark15 4 讨论 ···········································································································35 HYPERLINK \l _bookmark16 实验三 螺旋神经元损伤后 Wnt 通路分子的改变 37 HYPERLINK \l _bookmark17 1 材料 ·································