肺组织miRNA与非小细胞肺癌的关系-胸外科专业论文.docxVIP

硕士学位论文 硕士学位论文 万方数据 万方数据 摘 要 目的：通过对肺组织 miRNA 检测，探寻肺组织 miRNA 与非小细胞肺癌（Non Small Cell Lung Cancer，NSCLC）的关系，并通过临床样本验证，探讨其各种临床价值。 方法：通过分析既往检测非小细胞肺癌 miRNA 的研究结果，选出 7 种相关特异性 miRNA （miR-21、miR-31、miR-125b、miR-155、miR-205、miR-210、miR-375），作为候选 miRNA， 初步构建液相芯片检测体系。收集非小细胞肺癌患者及肺部良性病变患者的肺部病变组织、 病变旁组织和正常肺组织，利用液相芯片定量检测体系对这几种标本进行 miRNA 定量检测， 再进行临床分析。 成果：建立了检测非小细胞肺癌 miRNA 液相芯片系统。7 种相关特异性 miRNA 在非小细胞 肺癌组织中的表达均较高，在癌旁组织和肺良性病变组织的表达均较低。7 种相关特异性 miRNA 在肺组织的表达与性别、年龄及嗜烟时间无明显相关； miR-21、miR-205 在无淋巴 转移的非小细胞肺癌组织中的表达要低于有淋巴转移的非小细胞肺癌组织；miR-210 的表达 与肿瘤大小及侵袭范围呈负相关。 结论：建立了检测非小细胞肺癌miRNA液相芯片系统，它具有多种优点。7种相关特异性 miRNA在非小细胞肺癌组织中均呈特异性表达，它们有各自的临床意义。 关键词： miRNA；非小细胞肺癌；液相芯片 I Abstract Objective:Through the relationship between the lung tissue of miRNAs detection, it can explore the relationship between lung miRNAs and non small cell lung cancer(NSCLC). And through the verification, it can explore the various clinical value. Methods: Researched on the previous detection of non small cell lung cancer miRNAs results of data analysis, 7 kinds of specific miRNAs(miR-21, miR-31, miR-125b, miR-155, miR-205, miR-210, miR-375) were screened as candidates. MiRNAs liquid bead array detection system was established for the 7 miRNAs. Collect the patients diseased tissue, tissue adjacent to the lesion and normal lung tissue, who were lung cancer patients and patients with benign lesions. And then use the NSCLC miRNAs liquid bead array system for quantitative detection of miRNAs and clinical analysis. Results: The NSCLC miRNAs liquid bead array system was established. 7 kinds of specific miRNAs showed high expression in NSCLC tissue and low expression of the adjacent tissue and benign lesions tissue showed. There are no significant correlation among 7 miRNAs expression with sex, age and smoking time. MiR-21, miR-205 expression in the NSCLC tissue without lymph node metastasis in is lower than NSCLC tissue with lymph node metastasis. MiR-210 expression and tumor size and scope of the invasion were negative correlated. Conclu