典型赤潮藻类可培养藻际细菌的分子鉴定与藻菌关系研究-水生生物学专业论文.docx

暨南大学硕士毕业论文 暨南大学硕士毕业论文 PAGE PAGE VI Abstract Community composition and abundance of bacteria which isolated from the phycosphere of Gyrodinium instriatum, Skeletonema costatum, and Chattonella marina were studied in order to know the bacterial community composition in different algal growth stages. Meanwhile the inhibitory effects of bacteria on the growth of algal cells were studied. and revealed role of bacteria in the red tide generating and vanishing process. The purpose of the study is to understand the role of bacteria in in the initiation and collapse of algal blooms, and to provide some basis information in mechanisms of bloom occurrence and means of bloom controlling. Culturable bacteria were isolated from the different growth stages of G. instriatum, S. costatum and C. marina using gradient dilution method, and were identified based on sequences of 16S rDNA V3 region. Phylogenetic trees were constructed using sequences in this study and the closest related sequences from GenBank. The genetic distances were calculated. Meanwhile, bacteria in different growth stages of these algae were analyzed quantitatively. Results showed that 12 different strains of bacteria were isolated from G. instriatum, 7 strains from S. costatum and C. marina, respectively, which belonged to Alphaproteobacteria, Gammaproteobacteria, Bacteroidetes, and Actinobacteria. Alphaproteobacteria was the most diversified and abundant group, followed by Bacteroidetes and Actinobacteria. The bacterial community structures were similar within different algal species, but varied among growth stages. The species number of culturable bacteria was highest in the stationary stage, and the abundance peaked in early decline stage. Results suggested that bacterial community might play important roles in the growth, population competition, and bloom dynamics of algal cells. Results of the screen experiments showed that the bacteria strain Gi-1 isolated from G. instriatum had significantly inhibitory e