基因间长非编码rna与中国人群食管鳞状细胞癌发生发展的关联研究-遗传学专业论文.docxVIP

PAGE PAGE IV 基因间长非编码 RNA 与中国人群食管鳞状细胞癌发生发展的关联研究 英文摘要 The association between long intergenic non-coding RNA and the development of esophageal squamous cell carcinoma in Chinese populations Abstract Part Ⅰ LincRNA-uc002yug.2 Involves in Alternative Splicing of RUNX1 and Promotes Esophageal Cancer Progression It has been determined that long intergenic noncoding RNAs (lincRNAs) have critical regulatory roles in human cancer biology, however, the contributions of lincRNAs to esophageal squamous cell carcinoma (ESCC) have been infrequently explored. This study was to study the contribution of lincRNAs to ESCC by screening candidate lincRNAs in ESCC susceptibility loci that identified by multiple genome wide association studies (GWAS). So, we performed an independent two-centered study with consistent results in 684 patients diagnosed with ESCC from eastern and southern Chinese populations. We found that, among these candidate lincRNAs, only lincRNA uc002yug.2 was highly expressed (commonly 3.63 fold increase) in ESCC tissues compared to paired adjacent normal tissues, the expression level of lincRNA-uc002yug.2 was significantly associated with ESCC prognosis, and the prognosis of patients with high levels of lincRNA-uc002yug.2 were poor (HR=2.61; 95%CI=1.50-3.78). By further biochemical experiments, we studied the role of lincRNA-uc002yug.2 in ESCC progression. We found that lincRNA-uc002yug.2 might involve in the alternative splicing of RUNX1 gene through facilitating the affinity of splicing factor with RUNX1 to produce more RUNX1a with tumor promotion function, and further inhibit the tumor suppressor gene CEBPα, thereby promote cell proliferation and tumor growth. On the basis of above findings, we can know that lincRNA-uc002yug.2 may involve in 英文摘要 基因间长非编码 RNA 与中国人群食管鳞状细胞癌发生发展的关联研究 alternative splicing of RUNX1 to promote the high risk and poor prognosis of ESCC. Keywords: Esophageal squamous cell carcinoma; Prognosis; Long intergenic noncoding