互花米草生理生化特性对盐胁迫的响应-生物化学与分子生物学专业论文.docxVIP

Responses of physiological-biochemical characteristic of Spartina alterniflora to NaCl stress Postgraduate: Kang Hao Supervisor: Shi Gui-yu Grade: 2007 Major: Biochemistry and molecular biology Specialty: Chemistry of enzyme and protein Abstract This study was carried out to investigate the effect of NaCl stress on growth, the membrane lipid peroxidation, membrane permeability, chlorophyll content, photosynthetic rate(Pn)、transpiration rate(Tr)、 stomatal conductance (Gs)、carbon dioxide between cells (Ci), water use efficient(WUE),Vpdl A-PAR, A-Ci,dark respiratory rate, roots activities, specific leaf area(SLA) and leaf dry matter content(LDMC) of Spartina alterniflora, meanwhile it’s response to instant CO2 concentration changes, as well as it’s photosynthesis , transpiration and other physiological characteristics were deterred. The major results are summarized as follows: When NaCl concentration is higher than 500mmol/L, the height of S. alterniflora was inhibited fiercely, height could take as NaCl stress symptoms; however, leaf length and diameter is no significant difference, so they can’t be symptoms under NaCl stress. Chlorophyll content is inhibited fiercely (P0.05). Chlorophyll content was declined and membrane permeability was increased when NaCl concentration higher than 300mmol/L. Height and leaf length is inhibited fiercely (P0.05) also, so they can be taken as symptom under NaCl stress. Low NaCl concentration can lead to membrane lipid peroxidation. Lower NaCl concentration could decrease the MDA and peroxide content by some protection mechanism. When NaCl concentration is higher than 300mmol/L, NaCl stress can lead to membrane lipid peroxidation fiercely, destroy the protection mechanism and increase the MDA content in the leaves; but the MDA content decreases a little in the roots. SOD, CAT and MDA have the same change trend on the basic, it can conclude first increase then decline, and they are positive