短枝条红型红富士苹果芽变的生物学特性研究果树学专业论文.docxVIP

Study Study on Bionomics in a New Spur and Red Stripe of Fuji Apple Abstract This research took the red Fuji bud mutation tested matedal．Xinhongl#and Nagafu2#as control materials，through measuring and comparing its biological characteristics，growth and leaf anatomical structure and photosynthesis indexes to clear the excellent charactedsties of Red Fuii sports．and then by using ISSR molecular marker identitication technology to the bud mutation．The ISSR fingerprints of 1 3 samples were construeted and the genetic relationship was analyzed and identified．which laid reliable theoretical basis for the validation ofnew varieties．The main results were as follows： (1)The florescence and phenophase of branch and leaf growth had differences among three cultivars；there were some remarkable differences between the sports and Nagafu2#．For example，the bud sports internode length was significantly shortened and the leaf area was obviously increased．Its characteristies performance of spur was similar to Xinhon91#and the spur sports presented significant spur traits．The indicators were beaer than others cultivars，weighing 29 1．029 average。containing soluble solid content of 18．66％．soluble sugar content of 13．73％．Calculating these indexes comprehensively． thinking the bud mutation’s spur traitS were obvious and showing easily into flower．high fruit rate． excellent quality and other characteristics，the conclusion be drawn that the bud mutation was an excellent spur and red stripe type of variation materials． (2)From the ultrastructure of pollen indexes determination results be seen，the bud mutation of pollen grains was the 1argest．followed by Xinhongl#，the smallest was Nagafu2#．The bud sports of polar axis length and equatorial axis length was significantly higher than that of Nagafu2#．Pollen grain in three materials had some differences，smaller pore diameter less number of bud spots was not obvious；the number of large aperture Xinhongl#more obvious and had convoluted ornamentation