动、静脉移植骨髓间充质干细胞治疗急性期脑梗死模型的比较.pdfVIP

Abstract ABSTRACT Objective: To investigate the difference of GFAP 、VEGF 、Bcl-2 、Bax and TUNEL positive cells in the ischemia penumbra, the level of TNF- in serum, the score of mNSS, the count of body weight and the distribution of stem cells in organs between intravenous and intracarotid route for bone marrow derived mesenchymal stem cells in the treatment of MCAO 6 hours. To investigate the difference of intravenous and intracarotid delivery route and the mechanism of BMSCs in the treatment of MCAO Methods: 1. The middle cerebral artery occlusion model was conducted by modified intraluminal suture method. 2. The bone marrow derived mesenchymal stem cells were isolated, purified and cultured by whole bone marrow adherent culture and identified by flow cytometry. BMSCs were dyed with CM-dil for tracing. 3. Transplantion was operated at MCAO 6 hours by intravenous and intracarotid route respectively. 4. 7days after transplantation, the score of mNSS and the count of body weight were evaluated. 5. The level of TNF- αin serum was detected by ELISA. 6. The expression of GFAP 、VEGF 、Bcl-2 and Bax were detected by immunohistochemistry. 7. Cell apoptosis was detected by TUNEL staining. Results: 1. The expression of surface antigen CD90、CD29、CD106 、CD11b、CD34、 CD45 were respectively 95.7%、97.3%、52.7%、6.01%、2.95%、2.26%. 2. Compared with PBS group, the scores of mNSS was significantly decreased in the BMSCs groups, the difference between intracarotid and intravenous BMSCs group was not showed. Compared with intracarotid PBS group, the count of body V Abstra