克雷伯氏杆菌的甘油代谢振荡机理及发酵过程在线监测生物化工专业论文.docxVIP

克雷伯氏杆菌的甘油代谢振荡机理及发酵过程在线监测Study 克雷伯氏杆菌的甘油代谢振荡机理及发酵过程在线监测 Study on the oscillation mechanism and online monitoring of glycerol metabolism by Klebsiella peneumoniae Abstract As the most important chemical material，1,3-propanediol has widespread use in multiple areas including cosmetic．medicine etc．It attract considerable interest especially for it can synthesis polytrimethylene terephthalate(PTT)as the monomer．Compared to traditional chemical synthesis methods．the biological methods iS more environment—friendly and fulfills the sustainable development．Using high concentration of substrate during continuous fermentation is an effective method to enhance the conversion of substrate，product concentration and yield．However，with the high concentration of substrate or products or both of them 1cad to oscillation or polymorphic etc． This thesis iS aimed to discuss the main reason induce the oscillation during continuous fermentation of glycerol by K．peneumoniae．Firstly，we study on the oscillatory behavior of Kpeneumoniae in continuous culture induced by high concentration of glycer01．The glycerol was enhanced from 20∥L to 1 00 edL lead to inhibit the growth of cell，the state of fermentation shift into lag phase．Right now．the concentration of the toxic middle metabolites，3-hydropropionaldehyde(3-HPA)had climbed to 0．63 g／L then fell to 0．1 8∥L． Other metabolites such as 1，3-propanediol，acetic acid also reached iIS lowest point，after 20 h the high concentration of glycerol of adaptation．The metabolites including the C02， 1，3．propanediol，acetic acid，3-HPA，formic acid，succinic acid etc starts to oscillate．The main oscillation period of 6．5．7．5 h，and the oscillation state Can 1ast for more than l 40 h．Besides， 3-HPA has a shorter oscillation period of 3．4h．the change in the concentration of it during the oscillation state are from 0．1 3．O．78∥L．Compared the metabolic flux of the oscillation state and the stable state shows a higher productivity and specific production rate of metabo