一体式pcr激发探测系统的研究光学工程专业论文.docxVIP

AbstractPolⅥnemse Abstract PolⅥnemse chain reaction was invented by the United States Mullis in 1 985·It is a m01ecular bi0109Y technique for the in vitro amplification of specific DNA fragments·Based on the polymerase chain theory,PCR instrument was invented．PCR instrument develoPed through qualitative to quantitative process．Current PCR technology Can already compute DNA quantitatively in the reactants．Fluorescence detection system IS one of the core of the comp．onents to implement the process．It detects the PCR reaction by the changes fluorescence intensity． Firstlyin this paper,based on the fluorescent light principle and characteristics，we use fluorescence analysis and the characteristics of excitation and emission spectra m quantitatlVe PCR fluorescent reagents．On the basis of oblique fluorescence detection method，we preliminary design a multi．band real．time detection system with four channel·In this system， 、ve use a t11rough．hole LED as the excitation light source and the PIN photodiode as the detector．Besides this，we design four sets of filters to separate the band· Then according to the principle of non-imaging optics，we use simulation software o：t Tracepro to simulate and optimize the excitation and detection system，SO that to achleVe a11 ideal effect of the solar energy utilization．During this simulation，we build a model and set the light source and the material properties to achieve optimization· Finallv’experimental results prove the feasibility of this fluorescence detection system· In this experiment，we can detect fluorescence signal emitted from FAM，and find a linear relationship between the fluorescence intensity and the concentration of the reagents by di成rent fluorescence intensity from different concentration ratio of FAM． Key Words：Quantitative PCR．Fluorescence analysis，TracePro simulation，Solar energy utilization II 万方数据 硕士论文 硕士论文 一体式PCR激发探测系统的研究 目 录 摘 要 I Abstract． ． ．．． ． ．． ． ．． ． ．． ． ． ． ． ． ． ． ． ．．II 1．绪论 ．1 1．1研究背景及意义 l 1．1．1聚合