uuo模型大鼠肾间质纤维化相关炎症因子抗体芯片分析病理与病理生理学专业论文.docxVIP

出现增高或降低，其中Fractalkine、GM．CSF、IL．1a、IL．1p、IL．6、 出现增高或降低，其中Fractalkine、GM．CSF、IL．1a、IL．1p、IL．6、 IFN吖、LIX、Leptin、MCP．1、MIP．3a、TIMP．1和n婚．0【共12种炎 症因子随肾间质纤维化程度加重呈增高的趋势，尤以MCP．1、 Fractalkine和TIMP．1增高最为明显(PO．05)；IL-4和IL．10表现为 下降趋势(PO．05)；VEGF、CINC．2和CⅢC．3表现为先升高后降低 的变化趋势(Po．05)；CNTF和[3-NGF这2种炎症因子无明显变化。 用辛伐他汀治疗后第14天，与假手术组相比，模型组和治疗组肾组织 纤维化明显，治疗组的肾间质纤维化程度轻于模型组。结论：单侧输 尿管结扎可以迅速建立理想的肾脏细胞转分化和肾间质纤维化大鼠模 型，且肾间质纤维化程度随肾功能的减退进行性加重。抗体芯片检测 结果以MCP．1、Fractalkine和TIMP．1变化最为明显，说明这三种炎 症因子在肾间质纤维化进程中发挥的促纤维化作用最大。Fractalkine 作为我们筛选出的重要细胞因子之一，其机制和作用尚未被完全研究 清楚，而辛伐他汀可能通过抑制Fractalkine及其受体CX3CRl的表达， 延缓肾间质纤维化的进展。 关键词：抗体芯片；炎症因子；肾间质纤维化；单侧输尿管结扎 Antibody Antibody Chips Analysis of Renal Interstitial Fibrosis Related Inflammatory Factors in Rats with Unilateral Ureteral Obstruction Abstract：0bj ective：Antibody array technology was applied to the detection of overall changes of inflammatory factors in renal tissues when renal interstitial fibrosis was formed in rats，SO that more inflammatory factors related to this progression Was selected．After the further research， the role of inflammatory factors was comprehended in depth．Thus，it Was helpful to clarify the mechanism of renal interstitial fibrosis and then a new research direction of the clinical prevention and treatment of chronic kidney diseases was provided．Methods：50 CL SD male rats were randomly divided into sham operation group(SOR)and unilateral ureteral obstruction group(tmo)．5 rats of each group were randomized to be killed on the 5m，7m，1 1 m，1 4小and 2 1或day after the operation of unilateral ureteral obstruction；5 ml of blood Was sampled to monitor the changes of renal function，and the renal tissues were collected on the side of the afflicted part to observe the pathological changes of the obstructive kidneys．Here by the most representative time point was determined in the progression of renal interstitial fibrosis，and next the renal tissues at the abovementioned time were tested with the technology of antibody array of cytokines，and the dynamic ch