利用HPLC法测定水稻子粒主要黄酮类化合物-植物遗传资源学报.PDFVIP

植物遗传资源学报2012,13(5):896-900 Journal of Plant Genetic Resources 利用HPLC法测定水稻子粒主要黄酮类化合物 1 1 1 1 1 1 2 杜　 娟 ,杨　 涛 ,曾亚文 ,普晓英 ,杨树明 ,陈晓艳 ,贾　 平 1 2 ( 云南省农业科学院生物技术与种质资源研究所,昆明650223; 昆明田康科技有限公司,昆明650205) 　 　 摘要:通过建立水稻儿茶素、杨梅素、槲皮素和山奈酚含量的HPLC测定方法,研究水稻黄酮类化合物含量及组成差异,为 高黄酮水稻种质资源的定向改良提供理论依据。 本研究建立了利用HPLC法测定水稻黄酮类化合物含量的测定方法;水稻糙 米的黄酮化合物组成主要以儿茶素和山奈酚的形式存在,不含杨梅素和槲皮素,不同品种儿茶素和山奈酚组成比例及含量存 在差异;粳稻含有更丰富的儿茶素,有色稻山奈酚和儿茶素含量大于无色稻;糙米中含有较高的黄酮类化合物,而精米中不含 或少含黄酮类化合物。 　 　 关键词:HPLC法;黄酮类化合物;水稻;儿茶素;山奈酚 Determination of Flavonoid in Rice Grain by HPLC 1 1 1 1 1 1 2 DUJuan ,YANGTao ,ZENG Ya-wen ,PU Xiao-ying ,YANG Shu-ming ,CHEN Xiao-yan ,JIA Ping ( Biotechnology and Genetic Resources Institute,YunnanAcademy of Agricultural Sciences,Kunming 650223;1 2 Kunming Tiankang Technology Co. ,Ltd. ,Kunming 650205) Abstract:Analysis of the difference in major flavonoid contents and compositions by high performance liquid chromatography(HPLC)method to determine catechin,myricetin,quercetin,and kaempferol was conducted in rice grain,aiming at providing the theoretic basisfor directional improvement of high flavonoid germplasm resource.The HPLC method for determining flavonoid in rice grain was developed.The major flavonoid in brown rice was com- posed of catechin and kaempferol,but did not contain myricetin and quercetin.There was significant difference a- mong the proportions andcontents.Thecultivars(orlines)ofjaponicaricehadmorecatechin,whilethecoloredrice had moremyricetin andkaempferolthanuncoloredrice.Brownricecontainedmoreflavonoidthanmilledrice,while milled rice contained little or no flavonoid. Key words:HPLC method;Flavonoid;Rice;Catechin;Kaempferol 黄酮类化合物