久效磷对雄性金鱼的生殖毒性研究-海洋生物学专业论文.docxVIP

精子3h，对照组、0．01，0．10和1．00 精子3h，对照组、0．01，0．10和1．00 mg．L。1暴露组彗星尾部DNA含量分别为： 1．174-0．8l％、4．554-1．24％、14．60a：2．17％、17．50a：1．98％；彗星尾长分别为： 3．94+1．29I-tm 6．80：t：2．78“m、9．21+1．51I_tm和12．89a：3．091uIm；彗星尾矩分别为： O．39a：0．271．tm、0．70-士-0．38、1．90a：0．45、4．47士0．339m。久效磷的暴露导致精子彗星 尾长、尾部DNA含量和尾矩的增加，表明久效磷可以损伤金鱼精子的遗传物质。 本文在组织水平、生化水平和DNA水平上，通过对精巢超显微结构的观察、 特征性酶活性的检测和精子活力、DNA损伤的分析，首次提出了久效磷对雄性 金鱼的生殖毒性是通过损伤精巢支持细胞、Leydig氏细胞及精子线粒体结构，影 响了多种特征性酶活性，最终导致生精障碍的；久效磷还能降低精子运动力，损 伤精子DNA，可能造成对受精过程、胚胎发育和予代健康的危害。 关键词：久效磷、金鱼、生殖毒性、特征性酶、DNA损伤 II The The Reproduction Toxicity of Monocrotophos on Male Goldfish，Carassius Auratus Abstract Monoerotophos is one of the environment hormones，because it call induce the production of vitellogenin in male goldfish．However,very few reports about the reproduction toxicity of monocrotophos exist in the literature．So the gonadosomatic index(GSI)of male goldfish(Carassius auratus)under monocrotophos treatment was tested in this paper．The activities ofseveral characteristic anzyrfles in testis were then measured；the micro-and ultrasmaemre of testis were observed as well．The toxicity effect of monoerotophos to DNA and motility of sperm will8 evaluated via the investigation of the viability,the percentage and duration of motility of sperms by microscope，and the percentage of DNA in comet tail(Tail DNA％)，the tail leng：th(TL) and tail moment(TM)by comet assay of sperm comets．All the works aim at exploring the effect and mechanism ofreproduction toxicity ofmonocrotophos on male goldfish． The data might give useful information to toxicological research of environment hormonesonreproductionsystemofhuman． Exposure to monocrotophos led to the change of GSI and microstructure of male goldfish．After exposedtomonocrotophosofvarious concentration(0．01、O．10、1．00 nag·L-1)for 21 days，the GSI of adult male goldfish decreased gradually with the increase of concentration of monocrotophos．The GSI of male goldfish in exposure concentration of 1．00 mg·L～decreased significantly compared to the c