鹿茸再生干细胞成骨诱导微粒体培养体系的建立-吉林农业大学学报.PDF

吉林农业大学学报 2010, 32( 6) : 680~ 683 http :/ / xuebao.j lau. edu. cn Journal of Jilin A ricultural University Email :j lndxb@ vip . sina. com () 孙红梅, 杨福合, 邢秀梅, 刘琳玲, 赵海平, 李春义 中国农业科学院特产研究所, 吉林 132109 : 针对鹿茸再生活体研究周期长耗 大对动物损伤大等问题, 利用在培养液中加入TGF1地塞米 松抗坏血酸磷酸甘油等对鹿茸再生干细胞进行体外诱导, 成功建立了一种鹿茸再生干细胞体外诱导成骨 的培养体系, 模拟了鹿茸软骨内骨化的生长过程 : 鹿茸再生干细胞; 成骨; 微粒体培养 : S825; Q7 : A : 2010) Establishment of Micromass Culture System for Antler Stem Cell Induc tion Toward Osteoblast Differentiation in Vitros SUN Hongmei, YA NG Fuhe, XING Xiumei, LIU Linling, ZHAO Haiping, LI Chunyi Institute of Special Wild Economic Animal and Plant Science, CAAS . , Jilin 132019, China Abstract: In vivo experiment involve the problem of long cycle, high co t and great damage to animal. Therefore, in thi experiment, we e tabli hed a microma culture y tem to induce antler regeneration tem cell differentiation toward o teobla t in vitro by adding tran forming growth factor1 ( TGF1) , a corbate2pho phate, dexametha one, glycerol pho phate to the culture medium. By o doing, we ucce fully mimiced the in vivo proce of antler endochondral o ification, and laid the fundation for the re earch of antler biology, regeneration, o ification. Key words: antler tem cell; o ification; microma culture , , , , [ 6] , , , 2 cm/ d[ 12] , [ 68] ,