碱性成纤维生长因子基因修饰大鼠骨髓间充质干细胞的体外分化实验研究-外科学(普外科)专业论文.docxVIP

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碱性成纤维生长因子基因修饰大鼠骨髓间充质干细胞的体外分化实验研究-外科学(普外科)专业论文.docx

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5 5 (P=0.0000.01 ), there is significance difference. There is no difference of the vigor among 3 groups (P =0.0000.05), The objective group is 0.398±0.186, the general group is 0.392±0.189, the black control group is 0.394±0.243. Adjusting from morphological, The primary rBM-MSCs are likely trabs, after culturing the rBM-MSCs with bFGF for 28 days they are likely cobble-stone, and conjuncting closely-up, but there is no difference among the 3 groups . The objective group can get Dil-AC-LDL and express fluorescent , but little or none of other two groups After induction ( P=0.8670.05),the objective group is 0.518±0.227, the general group is 0.047±0.027, the black control group is 0.071±0.019. After induction the objective group is high than other two group among CD31,CD34 and FⅧ（P0.01）, CD31 ,the objective group is (22.73±5.42)%, the general group is (2.57±1.21)%, the black control group is (7.85±1.83)%;CD34, the objective group is (26.32±3.95)%, the general group is (2.41±0.97)%, the black control group is (8.93±1.37)%; FⅧ,the objective group is (30.34±6.62)%, the general group is (1.97±0.52)%, the black control group is (8.93±17.37)% . Conclusions： Getting rBM-MSCs by the way of density gradient centrifugation;rBM-MSCs modified with LV of bFGF can secrete bFGF ; there is no difference of the vigor after being transfected by LV;the rBM-MSCs modified by bFGF are better to differentiate into cells as VECs than other two groups. Key words： Basic fibroblast growth factor; Rat bone marrow vascular endothelial cells;Vascular endothelial cells; Lentivirus vector; Differentiate 1 1 英文缩写英汉对照表 (按英文字母字顺排列）英文缩写 bFGF 英文全称 Basic fibroblast growth factor 中文全称碱性成纤维生长因子 BM Bone marrow 骨髓 DECP Diethulpyrocarbonate 焦碳酸二乙脂 DGC Density gradient centrifugation 密度梯度离心 Dil-AC-LDL Dil-acetylated low-density lipoprotein 荧光标记低密度脂蛋白达尔伯克（氏）改良格尔（氏） DMEM Dulbeccos modified eagles medium 培养基 DMSO Dimathyl sulfoxide 二甲基亚砜 DNA Deosyribonucleic acid 脱氧核糖核酸 EDTA Ethylene diaminetetra-acetia acid 乙