碱性成纤维细胞生长因子对大鼠离体脂肪组织存活时间影响的研究骨科专业论文.docxVIP

摘要 目的： 探讨碱性成纤维细胞生长因子是否能延长离体脂肪存活时间，是否对脂肪组织 存活产生保护作用，提高脂肪移植存活率。 方法： 选用 SD 大鼠 72 只，随机分为 2 组，每组 36 只，于腹部取出脂肪组织，脂肪 组织分别浸泡于 2ug/LbFGF 及生理盐水，将浸泡的大鼠自体脂肪组织在半小时、1 小时、 2 小时时植入大鼠背部。分别在移植后 7 日，30 日时取出植入体内的脂肪组织。各组移 植物行 HE 染色及 CD34 免疫组化染色，观察脂肪存活情况。 结果： bFGF 浸泡 30 分钟组的移植体的血管密度与盐水浸泡 30 分钟组的血管密度无 统计学差异; bFGF 浸泡脂肪组织 1 小时及 2 小时移植体的血管密度高于同时间生理盐水 浸泡移植体组; bFGF 浸泡 1 小时及 2 小时脂肪组织移植体的血管密度与生理盐水浸泡 30 分组无统计学差异;生理盐水浸泡脂肪移植，30 分钟、1 小时、2 小时组之间移植脂肪 移植体的血管密度存在差异。 结论： 1.较短时间（30 分钟左右），无论如何处理脂肪组织，离体脂肪组织活性无明 显差异。 2.如果无保护因素保护离体脂肪组织，脂肪组织离体时间较长（1 小时），活性 可能会随时间逐渐下降。 3.碱性成纤维细胞生长因子能提高离体脂肪组织离体存活时间，一定程度上达 到保护离体脂肪作用。 关键词: 碱性成纤维细胞生长因子(bFGF)；脂肪移植； 微血管密度；存活时间 论文类型：A （应用研究） Abstract Purpose：Whether Basic fibroblast growth factor（bFGF）can prolong the survival time of fat transplantation. Whether it has effects to protect the survival rate of adipose tissue, and to improve the survival rate of fat transplantation. Methods：The research chooses 72 female SD rats，which are averagely divided into 2 groups randomly．1ml autogenous fat grafts were excised from bilateral inguinal areas of every SD rats．The first half groups, were are soaked in 2ug ／ml bFGF half an hour，an hour and two hour respectively, whereas the other half groups were in normal saline corresponding to each former group. Then all the rats were transplanted by the adipose tissue.7 days later, we compared half of the six groups transplanted tissue through staining with HE and CD34 immunohistochemical method.，Then 30 days later， we took out another half of the six groups transplanted tissue through staining with HE and CD34 immunohistochemical method. The microvessel density is measured by medical image analysis system． Results：The groups’ vascular density between the adipose tissue soaked in bFGF 30 minutes and soaked in saline soak 30 minutes are no statistical difference; The groups vascular density which the adipose tissue soaked in bFGF 1 hour and 2 hours was significantly more than the same time soaked in saline implant group; The groups vascular dens