胶体金免疫层析法快速定量检测猪尿中β2-受体激动剂残留的研究-农产品加工及贮藏工程专业论文.docxVIP

ABST ABSTRACT PAGE PAGE III ABSTRACT The traditional colloidal gold-based lateral flow immunoassay is only used to qualitatively or semi-quantitatively detection. The result is obtained according to the color of the test line, but it would be influenced by the environment and the operators. Colloidal gold-based lateral flow immunoassay system, that involves the use of a portable strip reader, can rapidly and quantitatively detect targets in samples according to the T/C ratio. It is rapid, convenient, sensitive, cheap, quantitative and suitable for on-site testing. In the research, it aimed to develop the colloidal gold-based lateral flow immunoassay for rapid quantitative detection system, and initially explore the stability of the system. The research will provide the theoretical basis and basic data for extending the application of the rapid quantitative detection system. Recently, clorprenaline has been found to be used as a new kind of “lean meat powder”. It metabolizes slowly and accumulates in animal bodies, the animal product will threat to the health of humankind by food chain. A competitive colloidal gold-based lateral flow immunoassay with a portable strip reader has been successfully developed for rapid quantitative detection of clorprenaline residue in swine urine. The detection system was optimized so that the detection can be completed within 9 min with the limit of detection of 0.15 μg/L. The assay exhibited good linear range from 3.0 μg/L to 20.0 μg/L with reliable correlation of 0.997 and with no obvious cross-reaction with five other β2-agonist compounds. Twenty spiked swine urine samples were tested by lateral flow immunoassay and LC–MS/MS to confirm the accuracy of the system. Results show good correlation between the two methods. This method is convenient, rapid, sensitive, and specific. It can be applied in the field for on-site detection of clorprenaline in urine samples. In the farmed animals industries, clenbuterol, ractopamine and salbut