庆祝叶朝辉教授荣膺中国科学院院士生物固态NMR-中山大学化学系.PPT

 Symposium of Frontiers in NMR Celebration of Professor Ye Chaohui for His Induction into The Chinese Academy of Sciences 生物固態NMR進展：奇半整數四極核之應用Recent Development in Biological Solid State NMR：Applications of Odd-Half-Integer Quadrupolar Nuclei 國立中山大學化學系 Department of Chemistry, National Sun Yat-sen University 丁尚武 Shangwu Ding 2002年4月8日於武漢 Wuhan April 8, 2002 Contents 1. Introduction 2. Principle of MQMAS 3. Example: ATP sodium salts 4. Summary 5. Concluding Remarks Acknowledgments Introduction The era of structural biology Why Solid state NMR for biomolecules? ----Liquid state NMR: fast motion ----X-ray: single crystal ----Microimaging: insufficient resolution ----Others: invisible to bulk spins Brief History Before 1980: unborn 1980s (S.J.Opella): Small molecules; Individual amino acids 1990s: C-13 chemical shift tensors of all 20 amino acids (Chaohui Ye et al., 1993.) N-15 CSA tensors amino acids: incomplete N-15 CSA tensors of four bases (ATGC), (D.M.Grant et al., 1998. Polypeptides and proteins (R.Griffin 1990, T.A.Cross et al., 1996; S.J.Opella et al., 1995, G.Drobny et al., 1995; R.Tycko et al., 1999, M.Hong, 1998; etc.) Ion-biomolecule interactions (J.Shaefer, 1997; A.E.McDermott, R.E.Wasylishen 1995; G.Wu; 1998 etc.) Major Missions Membrane-bound peptides and proteins Metalloproteins Ligand-receptor interactions Nucleic acids (DNA, RNA) Enzyme reaction mechanisms Protein folding/unfolding/misfolding MQMAS Principle (I) Coordinate Systems MQMAS Principle (II) Quantitative spectrum. 展望及建議Concluding Remarks 放鬆對樣品的要求 More tolerant to samples 更好地利用質子 Better use of protons 更多地利用四極核 More use of quadrupolar nuclei 動態研究 Dynamics 理論方法，計算模擬 Theory and computation 影像技術(量子分子顯微術？) Imaging techniques (Quantum Molecular Microscopy？) 應用 More extensive applications 強烈推薦：最好的學位論文方向或博士後研究課題 I strongly recommend