骨膦抗去势雌鼠骨质疏松研究-中国骨质疏松杂志.PDF

22 CHINESEJOURNALOFOSTEOPOROSISVol，3No ， 11997 骨膦抗去势雌鼠骨质疏松研究 郑承杰 季洪洋 付 乙 谭建三 摘要 切除8月龄雌性大鼠双侧卵巢后，皮F洼射骨瞵l2周，与单纯卵巢切除组 (OVX组)、假手术 组(sham组)及口服尼尔雌醇组(E 组)比较，用骨生物力学、骨密度测量、骨形态计量学等方法对骨膦的 药效进行综合评价 结果表明．与sham组比较，OVX组骨灰重、骨干重、骨钙含量及骨密度、抗弯力、骨小 粱体积密度、宽度均明显降低，而成骨细胞指数和破骨细胞指数、尿羟脯氯馥则明显增高。骨膦和E。对上 述指标有显著改善作用。 关t调 骨膦 卵巢切除 骨质疏松 骨生物力学 骨形态计量学 ExperlmenialstudyOllanti—o*teoporosiseffectofclodronateInovariectomizedfemaleRats ZhengChengjieLiHongyangFuYiandTanJiansan OccupationaldiseasesPreventionandTreatmentCentre WestChinaUniversityofMedicalSciencestChengdu610041 Al~traetInordertoevaluatetheant[-osteoporosiseffectofclodronateinovariectomizedofmalerats． 44femaleW istarratsaged8monthsweredividedrandomlyinto4groups：a)simpleovariectomizedthe (OVX)group；b)shamoperatedgroup；c)theE3groupwhichweresubjectedtobiLateralovarieetomyand treatedonthenextdaywithnilestrioLlmg／kgBW bygarageonceaweekfor12weekstandd)theclo— dronategroupwhichweresubjededtobilateraLovariectomyandtreatedonthenextdaywithclodronate 5mg／kgBW bysubcutaneousinjectiontwiceaweekfor12weeks．ALltheanimalsweresaerified12weeks aftertheoperation．ThebloodandurinesampleswerecollectedfordeterminationotbiochemicaIin— dices．Thehumeriandtibiaewerepreparedfordeterminationofnetweight，ashweight，calcium andphos— phoruscontent．bonedensity．asweLlasbonehistopathologyandhistomorpbometry-Theleftfemora。 re processedforboneantiCHTVepower．Theresultsshowedthatascomparedwiththesham group,thenet weight，ashweighttcalcium content，densityandbendingresistanceofbones，thetrabccularbonevoLume andthicknesswereallsignificantLyreducedintheOVXgroup，whileitsosteoblastindex，osteoc]astindex andurinehydroxyprolinecontentwereobviouslyincreased．Int