固定化酶法生产葡萄糖酸钠研究-微生物与生化药学（理）专业论文.docxVIP

ABSTRACTSodiumgluconatehasawiderallgeofapplicationsint11efood，constructionindustrialandtexturedyeiIlgsectors．CurrentlymereisanincreasingdemandforsodiuIngluconateinmemarkets．ProductionofsodiumgluconatebyenzymebiocatalysishaSattractedgreatattentionduetoitsrelatiVelylowcosta11dperceiVedenVironmentalbenefits．To舢曲ercutthecost，itisimportanttoreusetheeI亿ymecatalyst．In锄sstudy’meefrectofdif!fbremer亿ymeiIllmobilizationmemodsontheGODandCATaIldmeparameters疆-ectingsodiumgluconateproductione伍ciencywereinvestigated．E妇Fectsofdi丘．erentmemodsincludingcross-linkingofglu洲dehyde，sodiumalginateenn印mentandcoValemlyliI墩edwithepoxyresinsont11ee伍ciencyofimmbilizationwereinVestigated．’I’11emethodofcoValennyliI墩edwitllepoxyresinsshowedexcellentenzymeactiVit)，a11daCtiVi够recoVerywaschosenformnherimmobilizationstudy．TheoptimumconditionsfortlleirIullobilizationofGODandCATwereaSfollowS：ES-41g，锄。吼tsofGODaIldCAT，1．O“andO．8ml；pH6．5and6．O；sodiumphosphatebu仃er，bomare1．0M；tenlpe“船e，20℃and30℃；fixedtime，24hand32h．Enzymaticproper眵studiesshowthattheoptiIllaltemperaturea11dpHforimmobilizedGODwere38℃a11d6．5，respectiVely，whereaSfor行eeenzymetheoptimalconditions、Ⅳere32℃aIld6．0，respe“Vely．W1lentheenzymewasiIlcubatIedat60℃forlh，morethaIl80％a11d12％residualactiV时ofi删nobilizedGODa11d毹eenzymeremained，respectively．Zn2+eIlll觚cedtheir岫obilizedGoDactiv咄whilecu2+、A矿、H矿stronglyinllibitedmeactiVit)，．Storagestabili够testssho、№dtllat90．4％enzymaticactiVit)，ofimmobilizedGODwausretainedwhenstoredforseVenweeks．Theoptimumtemperatureofboth舶ea11di瑚mobilizedCATwereshoWntobe30℃．Theoptim眦pHoffreea11dimmobilizedCATwere6．8and7．0，respectively．Zn2+、Ca2+、Cu2+、Cr3+exKbitinllibito珂e虢ctsont11eimmobilizedCAT，aIldCu2+haSme舒eateste仃ect．ImmobilizedCATstillremained88．5％actiVi够aReritwaSstoredforseVenweeks．One-f-actorexperimentwaSc删edouttooptimizesodiumgluconateproductionTheoptiIIlumconditiollSwere：massratioofimmobilizedGODaIldCATisl：3，subs缸．ateconcen廿ation250g／L，temperature36℃，pH6．8，Ventilation0．8wm，reactiontime14h．ItwaSfoundthat70％aIld62％oftheiIlitial