双酶水解菜籽粕及水解物的抗氧化活性研究.pdfVIP

Vol.18, 2011, No.4 　　　　 Cerea l and F ood I nd us try 双酶水解菜籽粕及水解物的抗氧化活性研究 宋　寅, 胡　蓉, 严梅荣＊ 南京财经大学食 科学与工程学院　(南京　210003) 　:中性蛋白酶分别与alcalase, protamex, flavourzyme,木瓜蛋白酶及胰蛋白酶相结合, 用双酶相继水解菜籽粕, 得到5 组菜籽粕的双酶水解物。双酶水解物抗氧化活性试验结果表明,所 有水解物都具有 一定清除D H 自由基活性的能力、还原能力和抑制亚油酸氧化活性的能力, 但 以中性蛋白酶和胰蛋白酶组合的水解物最强。该水解物的凝胶层析分离结果表明, 4 组分离物中 分子量为6 500 ～1 050 Da 的组分清除D H 自由基的活性最高, 显示菜籽粕双酶水解物的抗氧 化活性取决于水解物的分子结构, 即不仅与酶的种类有关, 还与水解条件和水解度有关。 :菜籽粕;双酶;水解物;抗氧化活性;凝胶层析 + :TS225.1 4　　:A 　　:1672-5026(2011)04-0026-05 Study on dienzymatic hydrolysis of rapeseed meal and antioxidant activity of the hydrolysates ＊ Song Yin, Hu Rong, Yan Meirong College of Food Science and Engineering, Nanjing University of Finance and Economics (Nanjing 210003) Abstract:Five hydrolysates of rapeseed meal were prepared by hydrolysis with two enzymes in succession, including neutrase with alcalase, protamex, flavourzyme, papain and trypsin re- spectively .Antioxidant activity of the hydrolysates was tested.The results show that all five hy- drolysates show some degrees of D H radical-scavenging activity, reducing power and inhibition of linoleic acid peroxidation, and the hydrolysate from neutrase and trypsin was the strongest a- mong them.Four fractions of this hydrolysate were separated by gel filtration and the one with molecular weight between 6 500 ～1050 Da had the highest D H radical-scavenging activity.It is clear that the antioxidant activity of hydrolysates was dependent on molecular structure, which was not only relat