光谱研究知母活性组分芒果苷与蛋白质的相互作用-化学 分析化学专业论文.docxVIP

英文摘要Abstract 英文摘要 Abstract Investigating the interaction between drugs and proteins has much importance in pharmacokinetics and toxicology．The interaction call exert important effect on the distribution，free concentration，biological activity and metabolism of the drug in blood stream，and provide an important theoretical base on studying human disease， dosage forms and development of new drugs．In this dissertation,mangiferin,an active Component in Chinese Traditional Medicine Anemarrhena asphodeloides Bge， interacting wim Bovine Serum Albumin,Insulin and Glucagon Was studied．On the basis of the previous research,the following major innovative works were carried out： (1)Mangiferin was chosen to study its interaction to Serum Albumin and Peptides， and the quenching rates，binding constans in different systems were compared． (2)The interaction of mangiferin to insulin and glucagon was investigated in ternary system，the results indicated that the Peptides were competitive with each other to act on mangiferin． (3)The secondary structure compositions of insulin and glucagon were obtained by quantitative analysis using IR self deconvolution with second-derivative resolution enhancement and curve—fitting procedure． This dissertation consists of four chapters． Chapter l：The structures，functions and natures of proteins were briefly introduced firstly．The studying method，theory,and development of interaction between small ligands and proteins were summarized． Chapter 2：The mechanism of interaction between mangiferin(MA)and bovine serum albumin(BSA)in aqueous solution w嬲investigated by fluorescence spectra， synchronous fluorescence spectra,absorbance spectra and Fourier transform infrared (FT—IR)spectroscopy．The binding constants and binding sites of MA to BSA at Ⅱ 英文摘要different 英文摘要 different reaction times were calculated．And the distance between MA and BSA was estimated to be 5．20 nm based Off F6ster’s theory．Synchronous fluorescence and FT．IR measurements revealed that the se