含多糖菱角水提物的体外抗癌研究-药剂学专业论文.docxVIP

中文摘要多糖具有抗癌和免疫调节等多种药理作用，本论文对菱角壳、菱角肉中含多 中文摘要 多糖具有抗癌和免疫调节等多种药理作用，本论文对菱角壳、菱角肉中含多 糖的水提物分别进行提取、理化分析和定量分析，并对其体外抗肿瘤的活性进行 了测定。 以多糖的提取率为指标，分别讨论了温度、提取时间、加水量及pH值对菱 角多糖提取率的影响程度，并根据b(34)正交表确定最佳的提取工艺：温度80 ℃，加水量25倍，pH值为7，时间4h，提取3次。 对多糖水提物进行了脱蛋白，脱色等提纯处理，实验证明Ⅱ型ZTCI+I天然 澄清剂的脱蛋白效果优于Sevege法，D280强碱性阴离子大孔吸附树脂的脱色效 果优于双氧水脱色。提纯后多糖水提物性状为淡黄色，溶解性增加，费林反应显 示无还原糖，碘液反应呈现蓝色，pH值测定为5．02，熔点测定高于230℃。红外 测定，紫外鉴别与理化分析的结果都证实了菱角水提物含有多糖，凝胶色谱实验 结果显示菱角水提物为一组混合物，分子量分布较窄，同时，由蛋白测定、红外 图谱、紫外图谱以及凝胶色谱实验说明菱角水提物中可能含有糖蛋白或复合糖。 采用蒽酮．硫酸法测定菱角水提物中多糖的含量，采用3，5二硝基水杨酸法 测定菱角水提物中还原糖含量，结果表明，这组方法线性关系良好，加样回收率 和精密度都符合规定。 采用MTT法对菱角多糖水提物进行体外抗肿瘤实验的结果显示，菱角多糖 水提物对Hela细胞的抑制作用接近顺铂(P0．05)，多糖水提物在提纯后对Hela 细胞的抑癌活性反而降低，验证了中药起作用的协同性与复杂性。通过对两种乳 腺癌细胞的抑癌实验，同样显示多糖的抑癌作用与顺铂比较无显著性差异(P 0．05)。通过对小鼠皮肤成纤维细胞的实验，则显示多糖对正常细胞的细胞毒性 远低于顺铂(P0．01)。 本文的研究结果提示，含多糖的菱角水提物有可能开发成为一种抗肿瘤药 物。 关键词：菱角多糖水提物理化分析葸酮．硫酸法MTI肿瘤细胞 AB AB STRACT Polysaccharide has been suggested tO have anti·tunlor and imlilllne enhacing effects recently．ne aqueous extracts containing polysaccharides were extracted and purified from Trapa bispinosa Roxb，and their Physico-chemical properties were analysised in this study．The in、，i口o anti-tumor effect WaS also evaluated by MTT bioassay． An optimized extracting procedure WaS obtained through a L9(34)orthogonal experiment after evaluating the effects of temperature，duration of extraction，amOUnt of water added and pH values using the extraction rate of polysaccharide嬲the evaluation indicator．This optimized extracting condition Was finalized as temperature at 80℃，volume of water at 25 folds，duration of extraction at 4 hours and pH at 7．0， and the cycles of extracting procedures were repeated for 3 times． ne purification process for aqueous exracts includes the deproteinizafion and decolorization．Type II ZTCI+I natural clmfying agent was found better than Sevege method for deproteinization,and D280 anion large pore absorbing rasin was better than H202．The physico-chemical properties such as the appearance，the solubility,the reaction toward iodine，the pH，and the