凝胶色谱-超高效液相色谱测定动物油中的苯并（α）芘.pdfVIP

2016 年第 29 卷第 8 期 粮食与油脂 67 凝胶色谱- 超高效液相色谱测定 α 动物油中的苯并（ ）芘 康明芹，曹海微，田芳洁，邢燕燕 （吉林出入境检验检疫局，　吉林长春　130062 ） α 摘　要：建立了凝胶色谱– 超高效液相色谱（GPC–UPLC ）法测定动物油中苯并（ ）芘含量的方法。 动物油样品经丙酮– 乙腈（40 ∶60 ，V /V ）混合溶剂提取，凝胶色谱净化后，用超高效液相色谱仪 α μ 进行测定，外标法定量。苯并（ ）芘在2.0~50.0 g/L 的线性范围内线性关系良好，线性相关系数 为0.999 9。方法的检出限和定量限分别为0.3、1.0 μg/kg ，平均回收率为79.6%~104.8%，RSD 为 0.53%~2.86%。 α 关键词：凝胶色谱；超高效液相色谱；动物油；苯并（ ）芘 α Determination of benzo （ ）pyrene in animal oil by gel permeation chromatography-ultra performance liquid chromatography KANG Ming-qin ，CAO Hai-wei ，TIAN Fang-jie ，XING Yan-yan （Jilin Entry –Exit Inspection and Quarantine Bureau ，Changchun 130062 ，Jilin ，China ） α Abstract ：A GPC–UPLC method was established for the determination of benzo （ ）pyrene in animal α oil. The benzo （ ）pyrene was extracted by mixed reagent of acetone and acetonitrile （40 ∶60 ，V /V ）， cleaned up by GPC ，determined by UPLC and quantified by external standard method. The linear α μ correlation coefficient of benzo （ ）pyrene was 0.9999 in the range of 2.0~50.0 g/L. The limit of detection （LOD ）and the limit of quantitation （LOQ ）of the method were 0.3 ，1.0 μg/kg ，respectively. The average recoveries were 79.6%~104 .8% and the relative standard deviations （RSD ）were 0.53%~2.86%.