过量铁胁迫对豌豆幼苗毒害作用的研究-生物学 植物学专业论文.docxVIP

兰州大学硕士毕业论文 兰州大学硕士毕业论文 过量铁胁迫对豌豆幼苗毒害作用的研究 关键词：铁胁迫；豌豆；光合作用；叶绿素荧光；叶绿体膜；矿质元素；渗透调 解 2 兰州大学硕士毕业论文 兰州大学硕士毕业论文 过量铁胁迫对豌豆幼苗毒害作用的研究 ABS’I’量认C’l’ Iron toxicity is a widespread nutrient disorder caused by excess iron accumulation， especially in tropical and subtropical regions because of the lower redox potential in the acid soil．Iron toxicity Can seriously suppress the growth of plants and affect the production．Exploring the tolerance mechanism of the plants to iron toxicity can not only provide瑚some guidance in theory and practice to the plants growth in iron toxicity-prone areas，but also be the key to improving plants production to a large extent．In this study,the method of manual simulation soil FeZ+stress was used to study the toxic action caused by excess iron to the pea seedlings．111e major findings are as follows： 1．111e excessive iron caused the unnormal growth ofpea seedlings． The excessive Fe2+suppressed the normal growth of pea seedling obviously．First, the old leaves displayed some white and chlorosis spot while the growth of main roots Was supressed，which Was mainly manifested in the drop of total length, cross—sectional area and surface area ofpea roots．The last，the舶sh and dry weight of roots，stems，leaves dropped remarkably． 2．111e excessive iron obviously caused oxidative damage to biologicamacro-molecule in pea seedling． With the increase of iron concentration,the content of DNA and the protein dropped gradually while the MDA increased．More serious damage were observed in roots rather than in leaves． 3．The excessive iron decreased the leafs photosynthesis capacity obviously． The excessive Fe什decreased the net photosynthetic rate(Pn)、transpiration rate (Tr)、stomatal conductance(Os)、intercellular C02 concentration(Ci)、water use efficiency(WUE)and carboxylation efficiency(CE)gradually．At the same time,the PS II potential photochemical efficiency(Fv／Fo)、the P II maximal photochemical efficiency(Fv／Fm)、photosynthetic electron transport rate(ETR)、maximum quantu