基质金属蛋白酶9和白介素8在鼻咽癌中的表达及其与潜伏膜蛋白1的相关性研究-耳鼻咽喉科学专业论文.docxVIP

例。lO例鼻咽部慢性炎症常规石蜡切片标本也由浙江省温岭市第一人民医院病理 例。lO例鼻咽部慢性炎症常规石蜡切片标本也由浙江省温岭市第一人民医院病理 科提供，HE染色确诊为鼻咽部慢性炎症，其中男性6例，女性4例，作为对照组。 所有标本均经lO％中性甲醛固定，石蜡包埋，5um连续切片，用sP免疫组化法检 测MMP9、 IL8、LMPl的表达，采用SPSSl2．0统计软件对实验数据进行统计。 结果：1 MMP9的阳性表达率为66．04％(35／53)，平均阳性积分为33．19+29．73％； IL8的阳性表达率为66．04％(35／53)，平均阳性积分为33．46+30．23％： LMP!的阳性表达率为66．04％(35，53)，平均阳性积分为35．494-29．63％。 2 MMP9、IL8、LMPl的表达在颈淋巴结转移组中均显著高于无颈淋巴结 转移组(P0．05)，但在年龄、性别、病理类型、临床分期的分组中均 无显著性差异(PO．05)。 3 LMPl和MMP9的表达存在正相关(Y=O．792、p0．001)，LMPl和II．8 的表达也存在正相关(y=0．786、p0．001)。 结论：l MMP9、IL8、LMPl在鼻咽癌组织中均有广泛的表达。 2 MMP9、IL8、LMPI在鼻咽癌中的表达和鼻咽癌颈淋巴结转移存在显著 相关性，但和年龄、性别、病理类型、临床分期无相关性。 3 LMPI和MMP9、IL8的表达呈正相关，它可能通过多途径上调MMP9、 IL8的表达对鼻咽癌的转移起促进作用。 关键词基质金属蛋白酶9；白介素8；鼻咽癌；潜伏膜蛋白1 2 Expressions Expressions of matrix metalloproteinase 9 and interleukin 8 in nasopharygeai carejnoma and relationship with Epstein-Barr virus latent membrane protein-1 Graduate student Cai Zhaoyang Tutor Tang Jianguo Abstraet Background：Nasopharyngeal carcinoma(NPC)characterized with infiltrating and metastasis is one of malignant tumor in head and neck．90％NPC patients have been founded with neck lymphatic metastasis at their first examination．Studies have demonstrated the relationship between NPC and Epstein-Barr virus(EBV)infection． Latent membrane protein-1(LMPl)，expressed by EBV-DNA，is the primary carcinogen with multiple biological functions．Tumor metastasis is the result of multi— interaction between tumor cells and host cells．Newborn capillary blood vessels and basement membrane breakdown involved by tulnor cells have key role in tumor metastasis．Matrix metalloproteinase 9 (MMPg)is one of the representative substances that Can degrade basement membrane and extracellular matrix． Interleukin一8(IL一8)has been identified as aIl angiogenin，which play all important role in vascular formation in tumors．Previous studies had demonstrated that NPC can express MMP9，IL8 and LMP I highly,but their association with clinical data and correlation between each other were not very clear．In 0111experim