急性胰腺炎患者血浆NGAL测定及其临床意义-消化内科专业论文.docxVIP

III III Quantitative analysis of serum NGAL from patients with acute pancreatitis and its potential clinical significance Abstract Objective To quantify the serum neutrophil gelatinase-associated lipocalin (NGAL) from patients with acute pancreatitis and evaluate its potential clinical significance. Methods Blood samples collected from 43 patients with mild acute pancreatitis(MAP), 40 with severe acute pancreatitis(SAP), and 30 healthy controls were extracted for serum NGAL. The quantitation of serum NGAL was performed by sandwich enzyme-linked immunosorbent assay. The clinical association of NGAL level with scoring systems and biochemical parameter in acute pancreatitis was analyzed. Screening performance of the assay was calculated through the receiver operating characteristic(ROC)curve. Results The median concentration of circulating serum NGAL in SAP group was 362.97ng/ml, which was significantly higher than that in MAP group (143ng/ml, P0.001) and the healthy controls (34.23ng/ml, P0.001). The median concerntration of serum NGAL in patients with acute pancreatitis was higher than in healthy controls (P0.001).The area under the ROC curve performed by the serum NGAL from SAP and MAP groups was0.889 (95%CI 0.818—0.960,). With a cutoff value of 202.49ng/ml, the overall sensitivity was 85% and specificity was 84%. Serum NGAL level was found to be associated with APACHE-Ⅱscore (P0.001), Ranson score (P0.001), BISAP score(P0.001), CRP levels (P=0.014) and the length of hospitalization(P=0.002). Conclusion Serum NGAL level correlates with the extent of pancreatitis. It can be used in monitoring the development of acute pancreatitis and may be a potential marker for early diagnosis of severe acute pancreatitis. Key words: acute pancreatitis; serum NGAL;ELISA Written by: Jun Xu Supervised by: Wei-chang Chen； Su Yan Ab Abstract Quantitative analysis of serum NGAL from patients with acute pancreatitis and its potential clinical significance 4 4 目 录 H