姬松茸酚类物质的提取及其功能学的研究-农产品加工及贮藏工程专业论文.docxVIP

(4)以茶多酚为对照，研究了姬松茸酚的抑菌作用。结果表明：姬松茸酚 (4)以茶多酚为对照，研究了姬松茸酚的抑菌作用。结果表明：姬松茸酚 对金黄色葡萄球菌、大肠杆菌、枯草芽孢杆菌三种菌种均有抑制作用，但对试验 用的绿木霉和黑霉两种真菌抑制作用不明显。在一定温度内处理不影响姬松茸酚 的抑菌活性。姬松茸酚对金黄色葡萄球菌的最低抑菌浓度为6mg／mL，对大肠杆 菌的最低抑菌浓度为3mg／mL，对枯草芽孢杆菌的最低抑菌浓度为4mg／mL。 (5)利用高效液相法对姬松茸酚有效成分进行化学成分分析，在色谱条件 为柱温30℃；反相C18柱；运行130min；流速lmL／min；检测波长260nto下， 进行甲醇一水变梯度洗脱；可较好地分析姬松茸酚类物质中的各成分，并从中分 离出绿原酸。 关键词：姬松茸；酚；提取工艺；超临界C02萃取；抗氧化；抑茵；高效 液相 Ⅱ AbstractAgaricus Abstract Agaricus Blazei Murrill(hBM)belongs to Basidiomycotina,Hymenomycetes， Agaricales，Agaricaceae，and Agariceace．ABM is a kind of precious fungus used for food and medicine，which has rich nutrition and special health value．Extraction technology，the anti—oxidantive effect and the antibacterial activity of ABM polyphenols were studied in this paper．And then the active components of ABM polyphenols were analyzed by Waters 2695 HPLC．Therefore，all important recommedation for developing deep-processing products of ABM polyphenols were offered． (1)An extraction process of polyphenol from dried ABM was studied in this paper．Water and ethanol was used as the solvents and the material studied consisted of dried ABM．Different factors were considered，such as the concentration of solvent， extract temperature，material-water ratio，extract time，and frequency．And orthogonal tests were also done based on the single factor tests．It Was indicated that the optional conditions for organic reagents water extraction Was：the solidliquid ratio WaS 1：30， extracting temperature was 90C，extracting time 3 hours and the frequency was 3 times，the extraction rate could reach to I．584％．And the optional conditions for organic reagents ethanol extraction were：80％ethanol solvent Was used to extract polyphenols from the dried ABM by 1：25 under 60C three times and 2 hours for each time，the extraction rate could reach to 2．1 3 8％．Compared to the water extraction rate，the Ethanol extraction rate above the method of 35％．It Was indicated that the ABM polyphenols contented in the position of hut was 36％more than i